Food and Drug Control Laboratories, Ministry of Health, Tehran, Iran.
Toxicol Mech Methods. 2008;18(4):351-4. doi: 10.1080/15376510701610984.
ABSTRACT Oxytetracycline (OTC) is used for the prophylaxis and treatment of a great number of diseases since this antibiotic possesses broad-spectrum activity against many pathogenic organisms. The use of OTC has become a serious problem because of the possible existence of its residues in milk, which can be directly toxic or cause allergic reactions in some hypersensitive individuals. Even low-level doses of antibiotic in milk consumed for long periods can lead to problems regarding the spread of drug-resistant microorganisms. The purpose of the present study was to investigate residual OTC in consuming milk in Tehran using high-performance liquid chromatography (HPLC) with UV detector. OTC residues in extracts obtained from a preliminary cleanup procedure and recoveries from spiked OTC in desire concentrations were between 80% and 97% with appropriate coefficients of variation. The limit of detection (LOD) and limit of determination (LOQ) were 50 and 68.5 ng/mL, respectively. This result shows that this method would be useful for routine monitoring of oxytetracycline residues in bovine dairy milk.
摘要 土霉素（OTC）因其对许多致病生物体具有广谱活性，被广泛用于预防和治疗许多疾病。由于其在牛奶中的残留，可能会直接产生毒性或导致某些过敏个体发生过敏反应，因此该抗生素的使用已成为一个严重的问题。即使是长期摄入低水平剂量的抗生素也会导致耐药微生物传播的问题。本研究旨在使用带紫外检测器的高效液相色谱法（HPLC）检测德黑兰市消费牛奶中的残留土霉素。经初步清理程序提取的 OTC 残留量和期望浓度下添加 OTC 的回收率在 80%至 97%之间，变异系数适当。检测限（LOD）和定量限（LOQ）分别为 50 和 68.5ng/mL。结果表明，该方法可用于常规监测牛奶奶制品中土霉素残留。
