Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
Toxicol Mech Methods. 2004;14(6):339-43. doi: 10.1080/15376520490434692.
Nicotine, an active substance present in tobacco has been identified as a major risk factor for lung related diseases. In the present study, we have evaluated the protective effect of curcumin on circulatory lipid peroxidation and antioxidants in nicotine administered Wistar rats. Lung toxicity was induced by subcutaneous injection of nicotine at a dose of 2.5 mg/kg body weight (5 days a week, for 22 weeks) and curcumin (80 mg/kg body weight) was given simultaneously along with nicotine by intragastric intubation for 22 weeks. Enhanced lipid peroxidation (41.68%) in the circulation of nicotine treated animals was accompanied by a significant decrease in the levels of ascorbic acid, vitamin E, reduced glutathione, glutathione peroxidase, superoxide dismutase and catalase. Further, reduction in the levels of zinc and elevation of copper and ferritin were observed in circulation of nicotine treated rats. Administration of curcumin significantly lowered the concentrations of lipid peroxides (36%) and enhanced the antioxidant status with modulation in the levels of zinc, copper and ferritin. Our data suggest that curcumin exerts its preventive effects by modulating the degree of lipid peroxidation, antioxidant status and trace element levels.
尼古丁是烟草中存在的一种活性物质，已被确定为与肺部疾病相关的主要危险因素。在本研究中，我们评估了姜黄素对尼古丁给予的 Wistar 大鼠循环脂质过氧化和抗氧化剂的保护作用。通过皮下注射尼古丁（剂量为 2.5mg/kg 体重，每周 5 天，共 22 周）诱导肺毒性，并同时通过胃内插管给予姜黄素（80mg/kg 体重），共 22 周。尼古丁处理动物循环中增强的脂质过氧化（41.68%）伴随着抗坏血酸、维生素 E、还原型谷胱甘肽、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶水平的显著降低。此外，还观察到尼古丁处理大鼠循环中锌的水平降低，铜和铁蛋白的水平升高。姜黄素的给药显著降低了脂质过氧化物的浓度（36%），并通过调节锌、铜和铁蛋白的水平增强了抗氧化状态。我们的数据表明，姜黄素通过调节脂质过氧化、抗氧化状态和微量元素水平发挥其预防作用。
