Department of Biochemistry, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India.
Toxicol Mech Methods. 2005;15(6):375-81. doi: 10.1080/15376520500194783.
Nicotine, an active ingredient of tobacco smoke, is known to induce hyperlipidemia and disturb the prooxidant-antioxidant status. In our study, ferulic acid, a naturally occurring nutritional compound, was tested for its antioxidant and antihyperlipidemic property in a dose-dependent manner against nicotine-induced toxicity in female Wistar rats. We tested three different doses of ferulic acid-10 mg, 20 mg, and 40 mg/kg body weight-for their protective effects. The activities of biochemical marker enzymes lactate dehydrogenase and alkaline phosphatase, levels of peroxidative indices (thiobarbituric acid reactive substances and hydroperoxides), nitric oxide, and circulatory lipids (cholesterol, triglycerides, free fatty acids, and phospholipids) were increased significantly in the nicotine-treated group when compared to normal, which were brought down in ferulic acid-treated groups. The antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, vitamin E, and reduced glutathione) was found to be decreased in the nicotine-treated group, and was significantly increased in ferulic acid-administered groups. Further, ferulic acid also positively modulated the nicotine-induced changes in the micronutrients (zinc and copper) level. The dose 20 mg/kg body weight was found to be more effective than the other two doses. Our data suggest that FA exerts its preventive effects by modulating the degree of lipid peroxidation, antioxidant status, lipid profiles, and trace element levels during nicotine-induced toxicity.
尼古丁是烟草烟雾中的一种活性成分，已知它会引起高血脂，并扰乱氧化还原状态。在我们的研究中，测试了一种天然存在的营养化合物——阿魏酸，以其抗氧化和抗高血脂特性，针对尼古丁诱导的雌性 Wistar 大鼠毒性进行了剂量依赖性测试。我们测试了三种不同剂量的阿魏酸-10mg、20mg 和 40mg/kg 体重-以测试其保护作用。与正常组相比，尼古丁处理组的生化标志物酶乳酸脱氢酶和碱性磷酸酶的活性、过氧化指标（硫代巴比妥酸反应物质和过氧化物）、一氧化氮和循环脂质（胆固醇、甘油三酯、游离脂肪酸和磷脂）的水平显著升高，而在阿魏酸处理组中这些指标的水平则有所降低。抗氧化状态（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、维生素 E 和还原型谷胱甘肽）在尼古丁处理组中降低，而在阿魏酸给药组中显著增加。此外，阿魏酸还可正向调节尼古丁诱导的微量营养素（锌和铜）水平的变化。我们发现 20mg/kg 体重的剂量比其他两种剂量更有效。我们的数据表明，FA 通过调节脂质过氧化、抗氧化状态、脂质谱和微量元素水平，在尼古丁诱导的毒性中发挥其预防作用。
