免疫化学分析法检测与室内空气受串珠镰刀菌污染有关的 SAT-毒素 G 和其他大环多氧镰刀菌烯醇。
Immunochemical Assay for Satratoxin G and other Macrocyclic Trichothecenes Associated with Indoor Air Contamination by Stachybotrys chartarum.
机构信息
Department of Food Science and Human Nutrition and Institute for Environmental Toxicology, Michigan State University, East Lansing, Michigan, USA.
出版信息
Toxicol Mech Methods. 2003;13(4):247-52. doi: 10.1080/713857196.
Although satratoxin G (SG) is the primary macrocyclic trichothecene produced by Stachybotrys chartarum, a fungus frequently associated with outbreaks of indoor air illness, little is known about the actual quantities of this mycotoxin in suspect or normal building environments. Using antibodies produced against an SG hemisuccinate-bovine serum albumin conjugate, we devised an enzyme-linked immunosorbent assay that enabled the detection of as little as 100 pg/mL of SG and that could also detect other members of the satratoxin family. Detection by this assay of satratoxins in methanolic extracts of S. chartarum in rice cultures was comparable to that of high-performance liquid chromatography, indicating that this assay will be useful in detecting SG and other satratoxins in environmental samples.
虽然 satratoxin G（SG）是串珠镰刀菌（Stachybotrys chartarum）产生的主要大环三萜烯，该真菌常与室内空气疾病的爆发有关，但对于这种真菌毒素在可疑或正常建筑环境中的实际数量知之甚少。我们使用针对 SG 半琥珀酸-牛血清白蛋白缀合物产生的抗体，设计了一种酶联免疫吸附试验，该试验能够检测到低至 100 pg/mL 的 SG，并且还可以检测到 satratoxin 家族的其他成员。该试验通过对大米培养物中串珠镰刀菌甲醇提取物中的 satratoxins 的检测与高效液相色谱法相当，表明该试验将有助于检测环境样品中的 SG 和其他 satratoxins。
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