National Institute of Crop Science, Tsukuba 305-8518, Japan.
J Integr Plant Biol. 2009 Dec;51(12):1095-103. doi: 10.1111/j.1744-7909.2009.00886.x.
To understand the response of potato to salt stress, antioxidant enzyme activities and ion content were analyzed for a sensitive and a tolerant cultivar. Nodal cuttings of the tolerant cultivar, Kennebec, and the sensitive cultivar, Concord, were exposed to media without or with 30, 60, 90 or 120 mmol/L NaCl for 4 weeks. On exposure to NaCl, the length and fresh and dry weight of both shoots and roots of Concord showed greater decrease than those of Kennebec. The decrease in shoot growth was more severe than that of the root for both cultivars. The K(+) content of shoots and roots of both cultivars was reduced in a dose-dependent manner by exposure to NaCl; the Na(+) content increased. Activities of ascorbate peroxidase, catalase and glutathione reductase were increased in NaCl-exposed shoots of Kennebec; the corresponding activities in NaCl-exposed shoots of Concord were decreased. Roots of both cultivars showed similar changes in the activities of these enzymes on exposure to NaCl. These studies established that enzyme activities in Concord shoots are inversely related to the NaCl concentration, whereas those in Kennebec do not show a dose dependency, which is also the case for the roots of both cultivars. Our findings suggest that an increase in activity of antioxidant enzymes, such as ascorbate peroxidase, catalase and glutathione reductase, can contribute to salt tolerance in Kennebec, a salt resistant cultivar of potato.
为了理解马铃薯对盐胁迫的响应，分析了一个敏感品种和一个耐盐品种的抗氧化酶活性和离子含量。耐盐品种肯纳贝克（Kennebec）和敏感品种康科德（Concord）的节段插条在无盐或含 30、60、90 或 120mmol/L NaCl 的培养基中暴露 4 周。在盐胁迫下，康科德的地上部和地下部的长度、鲜重和干重的下降幅度均大于肯纳贝克。两种品种的地上部生长减少比地下部更为严重。盐胁迫导致两种品种的地上部和地下部的 K+含量呈剂量依赖性下降，而 Na+含量增加。盐胁迫下肯纳贝克地上部的抗坏血酸过氧化物酶、过氧化氢酶和谷胱甘肽还原酶的活性增加，而康科德盐胁迫下的相应活性降低。两种品种的根在暴露于 NaCl 时，这些酶的活性表现出相似的变化。这些研究表明，康科德地上部酶活性与 NaCl 浓度呈反比，而肯纳贝克则不呈剂量依赖性，两种品种的根也是如此。我们的研究结果表明，抗氧化酶（如抗坏血酸过氧化物酶、过氧化氢酶和谷胱甘肽还原酶）活性的增加有助于马铃薯耐盐品种肯纳贝克耐盐。
