IVF Centers Prof. Zech, Bregenz, Austria.
Reprod Biomed Online. 2009 Nov;19(5):700-7. doi: 10.1016/j.rbmo.2009.09.011.
During embryo vitrification, it is advisable that cooling and storage should occur in a carrier device in which there is complete separation of the embryos from liquid nitrogen to ensure asepsis. The consequence of a reduction in the cooling rate resulting from the heat-insulating barrier aseptic devices has to be counteracted by gradually increasing intracellular concentrations of cryoprotectants without inducing a toxic effect. Blastocysts originating from couples with male and/or female factor infertility (group 1) or from oocyte donors (group 2) or from in-vitro matured oocytes (group 3) were gradually exposed to increasing concentrations of dimethylsulphoxide/ethylene glycol (5/5%, 10/10% and 20/20%) before aseptic vitrification using a specially designed carrier (VitriSafe), a modification of the open hemi-straw plug device. A total of 120 aseptic vitrification/warming cycles were performed in group 1, 91 in group 2 and 22 in group 3. Survival rates before embryo transfer, ongoing pregnancy and implantation rates were as follows: for group 1, 73, 43 and 26%; for group 2, 88, 53 and 34%; and for group 3, 69, 50 and 38%, respectively. In spite of reduced cooling rates due to aseptic vitrification conditions, a three-step exposure to cryoprotectant solutions protects the embryos effectively from cryo-injuries and guaranties high survival rates.
在胚胎玻璃化冷冻过程中，建议在具有完全隔离的液氮载体设备中进行冷却和储存，以确保无菌条件。无菌隔热屏障导致冷却速率降低的后果，必须通过逐渐增加细胞内的冷冻保护剂浓度来抵消，而不会产生毒性作用。来自男性和/或女性因素不孕（第 1 组）或卵母细胞供体（第 2 组）或体外成熟卵母细胞（第 3 组）的囊胚逐渐暴露于二甲亚砜/乙二醇（5/5%、10/10%和 20/20%）的递增浓度下，然后使用专门设计的载体（VitriSafe）进行无菌玻璃化冷冻，这是开放半管塞装置的一种改良。第 1 组共进行了 120 次无菌玻璃化/解冻循环，第 2 组进行了 91 次，第 3 组进行了 22 次。胚胎移植前、持续妊娠和着床率如下：第 1 组分别为 73%、43%和 26%；第 2 组分别为 88%、53%和 34%；第 3 组分别为 69%、50%和 38%。尽管由于无菌玻璃化条件导致冷却速率降低，但三步暴露于冷冻保护剂溶液可有效地保护胚胎免受冷冻损伤，并保证高存活率。
