Larman Mark G, Hashimoto Shu, Morimoto Yoshiharu, Gardner David K
Vitrolife 3601 S. Inca St80110 Englewood CO USA.
IVF Namba Clinic 550-0015 Osaka Japan.
Reprod Med Biol. 2014 Feb 5;13(3):107-117. doi: 10.1007/s12522-014-0176-2. eCollection 2014 Jul.
The cryopreservation of gametes and embryos is vital to numerous fields of reproductive biology, including assisted human reproduction. With improved culture conditions, there are an increasing number of embryos to cryopreserve for potential use in subsequent cycles. Many of the gametes and embryos in human IVF are cryopreserved in open systems. Because liquid nitrogen is not sterile, concerns have been raised with regard to contamination from the liquid nitrogen and also cross-contamination between patients' germplasm. Human gamete and embryo cryopreservation are discussed, with recommendations on how to minimize and eliminate contamination, emphasizing the benefits of closed vitrification devices.
配子和胚胎的冷冻保存对于生殖生物学的众多领域至关重要，包括人类辅助生殖。随着培养条件的改善，需要冷冻保存以备后续周期使用的胚胎数量日益增加。人类体外受精中的许多配子和胚胎是在开放系统中冷冻保存的。由于液氮并非无菌，因此人们对液氮污染以及患者种质之间的交叉污染表示担忧。本文讨论了人类配子和胚胎的冷冻保存，并就如何尽量减少和消除污染提出了建议，重点强调了封闭式玻璃化装置的益处。
