Feng Dong-Fu, Chen Er-Tao, Li Xue-Yuan, Liu Yong, Wang Yang
Department of Neurosurgery, No. 3 People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Neurol Res. 2010 Jun;32(5):476-81. doi: 10.1179/016164110X12556180206158. Epub 2009 Dec 21.
Despite the widespread use of optic nerve injury models to simulate central nervous system injury, model protocols vary from laboratory to laboratory, making it difficult to directly compare findings between studies.
To standardize the optic nerve crush injury model, the commercially available Yasargil aneurysm clip, which provides a consistent clamping force, was used to produce a crush injury to the rat optic nerve. Histology was verified with hematoxylin-eosin. The number of retinal ganglion cells (RGCs) was counted by fluorescent gold dye labeling.
Following nerve crush injury, the density of RGCs was substantially reduced in the aneurysm clip-operated group relative to the normal and sham-operated groups, and no discernable difference was noted between the latter two control groups.
The present findings suggest that Yasargil aneurysm clip effectively produces permanent injury to the optic nerve with evidence from retrograde tracing of RGCs and may provide a standard technique for optic nerve crush studies.
尽管视神经损伤模型被广泛用于模拟中枢神经系统损伤，但不同实验室的模型方案各不相同，使得研究结果难以直接比较。
为了标准化视神经挤压伤模型，使用能提供一致夹力的市售Yasargil动脉瘤夹对大鼠视神经造成挤压伤。用苏木精-伊红染色验证组织学。通过荧光金染料标记计数视网膜神经节细胞（RGC）的数量。
神经挤压伤后，动脉瘤夹手术组的RGC密度相对于正常组和假手术组大幅降低，而后两个对照组之间未观察到明显差异。
目前的研究结果表明，Yasargil动脉瘤夹能有效地对视神经造成永久性损伤，有RGC逆行追踪的证据支持，并且可能为视神经挤压研究提供一种标准技术。
