Templeton Justin P, Geisert Eldon E
Department of Ophthalmology, Hamilton Eye Institute, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Mol Vis. 2012;18:2147-52. Epub 2012 Jul 27.
Our goal is to provide an instructional resource to help others wishing to use the optic nerve crush (ONC) as an experimental procedure. The process is described beginning with the anesthesia, followed by positioning of the mouse, the surgery itself, and post-surgical care. We analyzed the effect of ONC on retinal blood flow, using optical coherence tomography doppler. This procedure produces a consistent loss of cells in the ganglion cell layer, using whole mounts of retina stained with TO-PRO-3. An instructional video is presented that demonstrates a simple surgical approach to effectively crush the optic nerve of the mouse.
我们的目标是提供一种指导性资源，以帮助其他希望将视神经挤压（ONC）用作实验程序的人。该过程从麻醉开始描述，接着是小鼠的定位、手术本身以及术后护理。我们使用光学相干断层扫描多普勒分析了ONC对视网膜血流的影响。此程序使用用TO-PRO-3染色的视网膜整装片，在神经节细胞层产生一致的细胞损失。还展示了一个指导性视频，演示了一种简单的手术方法来有效挤压小鼠的视神经。
