Wang Xing-wen, Lin Xiao-yan, Bi Ying-hui, Han Jun-qing
Tumor center, Provincial Hospital affiliated to Shandong University, Jinan 250012, China.
Zhonghua Zhong Liu Za Zhi. 2009 Oct;31(10):742-5.
To evaluate the effect of actovegin (Nycomed, deproteinized hemoderivative of calf blood injection) on intestinal mucosa in rats with acute radiation enteritis, and observe the changes of expression of apoptosis-related bcl-2/bax genes.
An abdominal irradiation in a dose of 9.0 Gy X-ray of linear accelerator was performed once on a group of Wistar rats to establish a model of acute intestinal radiation enteritis. The experimental rats were randomly divided into five groups. Group 1 was normal control group; group 2 was model control group; groups 3, 4 and 5 were treated with low, middle and high dose of actovegin, respectively. After the model was established, actovegin injection was given intraperitoneally for successive 4 days. Corresponding intestinal tissues were taken for morphological examination with an image analysis system. The expression of apoptosis related bax and bcl-2 protein in the intestinal mucosal epithelial cells was determined by immunohistochemistry.
The groups 4 and 5 had significantly higher height of intestinal villi, the depth of crypt, the thickness of the mucosa and entire wall (254.66/261.71 microm, 166.47/165.41 microm, 510.44/511.71 microm, 610.38/608.98 microm), compared with those of the model control group (239.12 microm, 151.45 microm, 420.27 microm and 579.32 microm), respectively (P < 0.05). Treatment with middle and high doses of actovegin also significantly down-regulated the expression of activating apoptosis protein bax (24.54/23.24) compared with that of model control group (59.32) (P < 0.05) and up-regulated the expression of inhibiting apoptosis protein bcl-2 (55.54/52.21) compared with that of model control group (20.32) (P < 0.05). The ratio of bcl-2/bax was significantly higher in the groups 4 and 5 (2.2632, 2.1275) compared with that in the model control group (0.3425) (P < 0.01).
Actovegin accelerates the recovery of the acute radiation-injured intestinal mucosal epithelium by decreasing apoptosis via down-regulation of the expression of activating apoptosis protein bax and up-regulation of inhibiting apoptosis protein bcl-2.
评价爱维治（Nycomed，小牛血去蛋白提取物注射液）对急性放射性肠炎大鼠肠黏膜的影响，并观察凋亡相关bcl-2/bax基因表达的变化。
对一组Wistar大鼠一次性进行9.0 Gy直线加速器X射线腹部照射，建立急性肠放射性肠炎模型。将实验大鼠随机分为五组。第1组为正常对照组；第2组为模型对照组；第3、4、5组分别用低、中、高剂量的爱维治治疗。造模后，连续4天腹腔注射爱维治。取相应肠组织用图像分析系统进行形态学检查。采用免疫组织化学法检测肠黏膜上皮细胞中凋亡相关bax和bcl-2蛋白的表达。
与模型对照组（239.12μm、151.45μm、420.27μm和579.32μm）相比，第4组和第5组的肠绒毛高度、隐窝深度、黏膜厚度和肠壁全层厚度显著更高（分别为254.66/261.71μm、166.47/165.41μm、510.44/511.71μm、610.38/608.98μm）（P<0.05）。与模型对照组（59.32）相比，中、高剂量爱维治治疗也显著下调了促凋亡蛋白bax的表达（24.54/23.24）（P<0.05），上调了抑凋亡蛋白bcl-2的表达（55.54/52.21）（P<0.05）。与模型对照组（0.3425）相比，第4组和第5组的bcl-2/bax比值显著更高（2.2632、2.1275）（P<0.01）。
爱维治通过下调促凋亡蛋白bax的表达和上调抑凋亡蛋白bcl-2的表达来减少凋亡，从而加速急性放射性损伤肠黏膜上皮的恢复。
