Wang Xi, Cai Hongwei, Yan Xia, Ye Zhi
Department of Anesthesiology, Xiangya Hospital, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Apr;35(4):351-7. doi: 10.3969/j.issn.1672-7347.2010.04.012.
To determine the effect of different tidal volume(Vt) ventilation on apoptosis and the expression of Bax and Bcl-2 in rat lungs.
Twenty-four healthy SD rats were randomly divided into 4 groups: a control group, a low Vt ventilation group (LV), a middle Vt ventilation group (MV), and a high Vt ventilation group (HV). Rats were subjected to different tidal volumes (10,20,and 40 mL/kg) for 2 h except the control group, which kept their own breath. We determined the lung histopathology score, W/D ratio and WBC in bronchoalceolar lavage fluid (BALF) to evaluate the lung injury and examine the apoptotic cell death, Bax and Bcl-2 protein expression by using TUNEL technique and immunohistochemistry 24 h after the operation.
Compared with the control group, MV and HV increased lung histopathology score, W/D ratio, WBC in BALF, apoptosis index (AI )and Bax protein expression, but decreased Bcl-2 protein expression (P<0.05). These changes showed no significant difference between the control group and the low Vt ventilation group (P>0.05).
Low Vt ventilation contributed little to apoptosis. Higher Vt ventilation can improve Bax while inhibit Bcl-2 expression to aggravate apoptosis in rat lungs.
探讨不同潮气量（Vt）通气对大鼠肺组织细胞凋亡及Bax和Bcl-2表达的影响。
将24只健康SD大鼠随机分为4组：对照组、低潮气量通气组（LV）、中潮气量通气组（MV）和高潮气量通气组（HV）。除对照组自主呼吸外，其余各组大鼠分别接受不同潮气量（10、20和40 mL/kg）通气2小时。术后24小时，测定肺组织病理评分、肺湿/干重比（W/D）及支气管肺泡灌洗液（BALF）中的白细胞计数（WBC）以评估肺损伤，并采用TUNEL技术和免疫组化法检测细胞凋亡、Bax和Bcl-2蛋白表达。
与对照组相比，MV组和HV组肺组织病理评分、W/D、BALF中WBC、凋亡指数（AI）及Bax蛋白表达升高，Bcl-2蛋白表达降低（P<0.05）。对照组与LV组间上述指标差异无统计学意义（P>0.05）。
低潮气量通气对细胞凋亡影响较小。较高潮气量通气可上调Bax表达，下调Bcl-2表达，加重大鼠肺组织细胞凋亡。
