Wen Zhan-bo, Zhao Jian-jun, Li Jin-song, Wang Jie, Lu Jian-chun, Li Na
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, National Research Center for Bio-protection Equipment, Beijing 100071, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2009 Aug;43(8):686-9.
To establish a testing and evaluating method for filtration efficiency of the canister against microbial aerosol.
Serratia marcescens aerosol served as model of bacterial aerosol, Bacillus subtilis var niger aerosol as model of spores aerosol, bacteriophage f(2) aerosol as model of viral aerosol. Employing the microbial aerosol testing platform was established in lab, models of microbial aerosol generated artificially were sampled quantitatively by air samplers before and after filtrating by canisters, respectively. Filtration efficiency was determined by the concentration of microbial aerosol in the air sample before and after filtrating. The four canisters of 1-1, 1-2, 1-3, 1-4 were tested for the filtration efficiency against Serratia marcescens, Bacillus subtilis var niger and phage f(2) aerosol. The two canisters of 543 and 544 canisters equipped with active carbon were tested for the filtration efficiencies against Serratia marcescens aerosol.
The filtration efficiency of 1-1, 1-2, 1-3 canisters against Serratia marcescens, Bacillus subtilis var niger and phage f(2) aerosol was 100.000%. The filtration efficiency of 1-4 canister filtration efficiency against Bacillus subtilis var niger spores aerosol was 99.997% and efficiency of the other two aerosol was 100.000%. The filtration efficiency of the two canisters of 543 and 544 to those attached with active carbon against Serratia marcescens aerosol was 100.000%.
The testing method might be used to evaluate the protective performance of the canister against microbiological aerosol. The effect of the canisters (including those equipped with active carbon) against microbiological aerosol should be reliable.
建立滤罐对微生物气溶胶过滤效率的检测与评价方法。
以粘质沙雷氏菌气溶胶作为细菌气溶胶模型,枯草芽孢杆菌黑色变种气溶胶作为孢子气溶胶模型,噬菌体f(2)气溶胶作为病毒气溶胶模型。在实验室建立微生物气溶胶检测平台,分别对人工生成的微生物气溶胶模型在经滤罐过滤前后用空气采样器进行定量采样。根据过滤前后空气样本中微生物气溶胶的浓度确定过滤效率。对1-1、1-2、1-3、1-4四个滤罐进行对粘质沙雷氏菌、枯草芽孢杆菌黑色变种和噬菌体f(2)气溶胶的过滤效率测试。对543和544两个装有活性炭的滤罐进行对粘质沙雷氏菌气溶胶的过滤效率测试。
1-1、1-2、1-3滤罐对粘质沙雷氏菌、枯草芽孢杆菌黑色变种和噬菌体f(2)气溶胶的过滤效率为100.000%。1-4滤罐对枯草芽孢杆菌黑色变种孢子气溶胶的过滤效率为99.997%,对其他两种气溶胶的效率为100.000%。543和544两个装有活性炭的滤罐对粘质沙雷氏菌气溶胶的过滤效率为100.000%。
该检测方法可用于评价滤罐对微生物气溶胶的防护性能。滤罐(包括装有活性炭的滤罐)对微生物气溶胶的防护效果可靠。