[COX-2与VEGF在人肾透明细胞癌中的表达及其与血管生成的相关性]

[Expression of COX-2 and VEGF and their correlation with angiogenesis in human clear cell renal cell carcinoma].

作者信息

Chang Bao-dong, Cao Lin-sheng, Zhou Hui-liang

机构信息

Department of Urology, the Third People's Hospital of Zhengzhou city, Zhengzhou, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2009 Sep;31(9):687-90.

PMID:20021866

Abstract

OBJECTIVE

To investigate the expression of COX-2 and VEGF in clear cell renal cell carcinoma (CCRCC) and their correlation with tumor angiogenesis.

METHODS

Envision immunohistochemistry was used to determine the expression of COX-2 and VEGF, and microvessel density (MVD) was marked by CD34 in 80 CCRCC tissues and 20 normal kidney tissues. The relationship between the above mentioned markers were analyzed.

RESULTS

Expressions of COX-2 and VEGF were noted in both CCRCC and normal kidney tissues. The positive rates of COX-2 and VEGF were significantly higher in CCRCC than in normal kidney (P < 0.05); The expression of COX-2 was correlated with TNM stage (P < 0.05), histological grade (P < 0.05) and lymph node metastasis (P < 0.05) in CCRCC, but not with age (P = 0.663) and diameter of tumor (P = 0.528). Both COX-2 expression (r = 0.851, P < 0.01) and VEGF expression (r = 0.736, P < 0.01) were significantly associated with MVD in CCRCC. There was a positive correlation between expression of cox-2 and that of VEGF in CCRCC.

CONCLUSION

COX-2 expression is correlated with tumor angiogenesis in CCRCC. It is likely that VEGF is one of the most important mediators in the COX-2 angiogenic pathway.

摘要

目的

探讨环氧化酶-2（COX-2）和血管内皮生长因子（VEGF）在肾透明细胞癌（CCRCC）中的表达及其与肿瘤血管生成的相关性。

方法

采用Envision免疫组化法检测80例CCRCC组织和20例正常肾组织中COX-2和VEGF的表达，并用CD34标记微血管密度（MVD）。分析上述指标之间的关系。

结果

COX-2和VEGF在CCRCC组织及正常肾组织中均有表达。CCRCC组织中COX-2和VEGF的阳性率显著高于正常肾组织（P<0.05）；CCRCC组织中COX-2的表达与TNM分期（P<0.05）、组织学分级（P<0.05）及淋巴结转移（P<0.05）相关，但与年龄（P=0.663）和肿瘤直径（P=0.528）无关。CCRCC组织中COX-2表达（r=0.851，P<0.01）及VEGF表达（r=0.736，P<0.01）均与MVD显著相关。CCRCC组织中COX-2与VEGF的表达呈正相关。