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相似文献

1
The gross architecture of an antibody-combining site as determined by spin-label mapping.通过自旋标记图谱测定的抗体结合位点的总体结构。
Biochem J. 1977 Aug 1;165(2):177-97. doi: 10.1042/bj1650177.
2
Comparison of the dimensions of the combining sites of the dinitrophenyl-binding immunoglobulin A myeloma proteins MOPC 315, MOPC 460 and XRPC 25 by spin-label mapping.通过自旋标记图谱比较二硝基苯基结合免疫球蛋白A骨髓瘤蛋白MOPC 315、MOPC 460和XRPC 25结合位点的尺寸。
Biochem J. 1977 Aug 1;165(2):199-206. doi: 10.1042/bj1650199.
3
The combining site of the dinitrophenyl-binding immunoglobulin A myeloma protein MOPC 315.二硝基苯基结合免疫球蛋白A骨髓瘤蛋白MOPC 315的结合位点。
Biochem J. 1977 Aug 1;165(2):207-23. doi: 10.1042/bj1650207.
4
Antibody--hapten interactions in solution.溶液中的抗体 - 半抗原相互作用。
Philos Trans R Soc Lond B Biol Sci. 1975 Nov 6;272(915):53-74. doi: 10.1098/rstb.1975.0070.
5
Specificity of interactions of hapten side chains with the combining site of the myeloma protein MOPC 315.半抗原侧链与骨髓瘤蛋白MOPC 315结合位点相互作用的特异性
Biochem J. 1977 Aug 1;165(2):227-35. doi: 10.1042/bj1650227.
6
Interactions of the lanthanide- and hapten-binding sites in the Fv fragment from the myeloma protein MOPC 315.骨髓瘤蛋白MOPC 315的Fv片段中镧系元素结合位点与半抗原结合位点的相互作用。
Biochem J. 1976 Apr 1;155(1):37-53. doi: 10.1042/bj1550037.
7
Resonance Raman-spectroscopic studies of the hapten features involved in the binding of 2,4-dinitrophenyl haptens by the mouse myeloma proteins MOPC 315 and MOPC 460.对小鼠骨髓瘤蛋白MOPC 315和MOPC 460与2,4-二硝基苯基半抗原结合所涉及的半抗原特征进行共振拉曼光谱研究。
Biochem J. 1978 Nov 1;175(2):727-35. doi: 10.1042/bj1750727.
8
Structure of an antibody combining site by magnetic resonance.通过磁共振解析抗体结合位点的结构。
Nature. 1977 Mar 3;266(5597):31-7. doi: 10.1038/266031a0.
9
The binding of 2,4,6-trinitrophenyl derivatives to the mouse myeloma immunoglobulin A protein MOPC 315.2,4,6-三硝基苯基衍生物与小鼠骨髓瘤免疫球蛋白A蛋白MOPC 315的结合
Biochem J. 1978 Jan 1;169(1):179-88. doi: 10.1042/bj1690179.
10
Structural studies in solution on the combining site of the myeloma protein MOPC 315.骨髓瘤蛋白MOPC 315结合位点的溶液结构研究
Contemp Top Mol Immunol. 1977;6:1-52. doi: 10.1007/978-1-4684-2841-4_1.

引用本文的文献

1
Homogeneous antibody fragment conjugation by disulfide bridging introduces 'spinostics'.通过二硫键连接使均一抗体片段缀合引入“自旋诊断学”。
Sci Rep. 2013;3:1525. doi: 10.1038/srep01525.
2
Specificity of interactions of hapten side chains with the combining site of the myeloma protein MOPC 315.半抗原侧链与骨髓瘤蛋白MOPC 315结合位点相互作用的特异性
Biochem J. 1977 Aug 1;165(2):227-35. doi: 10.1042/bj1650227.
3
Crystallization of the Fv fragment of mouse myeloma protein M315.小鼠骨髓瘤蛋白M315的Fv片段结晶
Biochem J. 1979 Aug 1;181(2):497-9. doi: 10.1042/bj1810497.
4
Comparison of the dimensions of the combining sites of the dinitrophenyl-binding immunoglobulin A myeloma proteins MOPC 315, MOPC 460 and XRPC 25 by spin-label mapping.通过自旋标记图谱比较二硝基苯基结合免疫球蛋白A骨髓瘤蛋白MOPC 315、MOPC 460和XRPC 25结合位点的尺寸。
Biochem J. 1977 Aug 1;165(2):199-206. doi: 10.1042/bj1650199.

本文引用的文献

1
Kinetic mapping of the antibody combining site by chemical relaxation spectrometry.通过化学弛豫光谱法对抗体结合位点进行动力学图谱分析。
Biochemistry. 1974 May 7;13(10):2210-22. doi: 10.1021/bi00707a030.
2
An active antibody fragment (Fv) composed of the variable portions of heavy and light chains.一种由重链和轻链可变部分组成的活性抗体片段（Fv）。
Biochemistry. 1973 Mar 13;12(6):1130-5. doi: 10.1021/bi00730a018.
3
Localization of antibody-combining sites within the variable portions of heavy and light chains.抗体结合位点在重链和轻链可变区的定位。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2659-62. doi: 10.1073/pnas.69.9.2659.
4
Stereospecificity of murine myeloma protein-315 to enantiomeric spin labeled dinitrophenyl hapten.小鼠骨髓瘤蛋白-315 对映体自旋标记二硝基苯基半抗原的立体特异性
Immunochemistry. 1974 Jul;11(7):377-9. doi: 10.1016/0019-2791(74)90191-8.
5
Shape of the hydrophobic barrier of phospholipid bilayers (evidence for water penetration in biological membranes).磷脂双分子层疏水屏障的形状（生物膜中水渗透的证据）
J Membr Biol. 1974;15(2):159-92. doi: 10.1007/BF01870086.
6
Structural properties of the ligand binding sites of murine myeloma proteins.小鼠骨髓瘤蛋白配体结合位点的结构特性。
FEBS Lett. 1973 Apr 1;31(1):80-4. doi: 10.1016/0014-5793(73)80077-8.
7
Rotational correlation time of spin-labeled alpha-chymotrypsin.自旋标记α-糜蛋白酶的旋转相关时间。
Biochem Biophys Res Commun. 1972 Jan 14;46(1):321-7. doi: 10.1016/0006-291x(72)90665-1.
8
Magnetic resonance studies of spin-labeled creatine kinase system and interaction of two paramagnetic probes.自旋标记肌酸激酶系统的磁共振研究及两种顺磁探针的相互作用。
Proc Natl Acad Sci U S A. 1969 Sep;64(1):219-26. doi: 10.1073/pnas.64.1.219.
9
Spin-labeling as a general method in studying antibody active site.自旋标记作为研究抗体活性位点的通用方法。
Arch Biochem Biophys. 1969 Jan;129(1):296-307. doi: 10.1016/0003-9861(69)90179-9.
10
A spin-labeled hapten.一种自旋标记的半抗原。
Proc Natl Acad Sci U S A. 1965 Dec;54(6):1785-91. doi: 10.1073/pnas.54.6.1785.

通过自旋标记图谱测定的抗体结合位点的总体结构。

The gross architecture of an antibody-combining site as determined by spin-label mapping.

作者信息

Sutton B J, Gettins P, Givol D, Marsh D, Wain-Hobson S, Willan K J, Dwek R A

出版信息

Biochem J. 1977 Aug 1;165(2):177-97. doi: 10.1042/bj1650177.

DOI:10.1042/bj1650177
PMID:200219
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164889/
Abstract
  1. A series of Dnp (dinitrophenyl) nitroxide spin labels was used to map the dimensions of the combining site of the Dnp-binding immunoglobulin A myeloma protein MOPC 315. The method compares the observed e.s.r. (electron-spin-resonance) hyperfine splittings with those calculated on the basis of different postulated motions for the spin label. The analysis is complicated by the sensitivity of the e.s.r. hyperfine splitting to the overall ;tumbling' time of the antibody-hapten complex and the polarity of the spin-label's environment. When these effects are considered quantitatively, it is then possible to determine the degree of mobility of each hapten which is allowed by the shape of the combining site. 2. The dinitrophenyl ring is rigidly held, and the depth of the site is 1.1-1.2nm and has lateral dimensions at the entrance to the site >/=0.6nmx0.9nm. The analysis of the results for spin-labelled haptens with chiral centres allows these lateral dimensions to be refined to 0.8nm and 1.1nm, and it is shown that the site is asymmetric with respect to the plane of the dinitrophenyl ring. 3. A polarity profile of the combining site was also obtained and a positively charged amino acid residue, possibly arginine-95(L) (light chain), was located at the entrance to the site. 4. The binding of Gd(III) to the antibody-hapten complexes results in quenching of the e.s.r. signal of the nitroxide. By using La(III) as a control, the paramagnetic contribution to the quenching is measured. 5. Analysis of the differential quenchings of the enantiomers of two five-membered nitroxide ring spin labels gives two possible locations of the metal-binding site. One of these is equidistant (0.7nm) from each of the three dinitrophenyl aromatic protons, and nuclear-magnetic-resonance relaxation studies, at 270MHz, on solutions of dinitrobenzene, Gd(III) and the Fv fragment (variable region of heavy and light chain) from protein MOPC 315 support this location for the metal site. 6. The e.s.r. and metal-binding data were then compared with the results of a model of the combining site constructed on the basis of framework invariance in immunoglobulins [Padlan, Davies, Pecht, Givol & Wright (1976) Cold Spring Harbor Symp. Quant. Biol.41, in the press]. The overall agreement is very good. Assignments of possible chelating groups for the metal can be made.
摘要

  1. 一系列二硝基苯基（Dnp）氮氧自由基自旋标记物被用于描绘Dnp结合免疫球蛋白A骨髓瘤蛋白MOPC 315结合位点的尺寸。该方法将观察到的电子自旋共振（e.s.r.）超精细分裂与基于自旋标记物不同假定运动计算出的结果进行比较。由于e.s.r.超精细分裂对抗体 - 半抗原复合物的整体“翻滚”时间以及自旋标记物环境的极性敏感，分析变得复杂。当对这些影响进行定量考虑时，就有可能确定结合位点形状所允许的每个半抗原的移动程度。

  2. 二硝基苯基环被牢固固定，位点深度为1.1 - 1.2纳米，位点入口处的横向尺寸≥0.6纳米×0.9纳米。对手性中心的自旋标记半抗原结果的分析使这些横向尺寸精确到0.8纳米和1.1纳米，并且表明该位点相对于二硝基苯基环平面是不对称的。

  3. 还获得了结合位点的极性分布图，并且在该位点入口处发现了一个带正电荷的氨基酸残基，可能是精氨酸 - 95（轻链）。

  4. Gd(III)与抗体 - 半抗原复合物的结合导致氮氧化物的e.s.r.信号淬灭。通过使用La(III)作为对照，测量了顺磁对淬灭的贡献。

  5. 对两种五元氮氧自由基环自旋标记物对映体的差异淬灭分析给出了金属结合位点的两个可能位置。其中一个与三个二硝基苯基芳族质子中的每一个的距离相等（0.7纳米），并且在270MHz下对二硝基苯、Gd(III)和来自蛋白MOPC 315的Fv片段（重链和轻链可变区）溶液进行的核磁共振弛豫研究支持了该金属位点的这个位置。

  6. 然后将e.s.r.和金属结合数据与基于免疫球蛋白框架不变性构建的结合位点模型的结果进行比较[帕德兰、戴维斯、佩奇特、吉沃尔和赖特（1976年）《冷泉港定量生物学研讨会论文集》41卷，即将发表]。总体一致性非常好。可以确定金属可能的螯合基团。