小鼠骨髓瘤蛋白M315的Fv片段结晶
Crystallization of the Fv fragment of mouse myeloma protein M315.
作者信息
Aschaffenburg R, Phillips D C, Rose D R, Sutton B J, Dower S K, Dwek R A
出版信息
Biochem J. 1979 Aug 1;181(2):497-9. doi: 10.1042/bj1810497.
Abstract
The Fv fragment of mouse myeloma protein M313 was crystallized from poly(ethylene glycol) solution in the form of monoclinic crystals, space group C2 and unit cell dimensions a = 5.96 nm (59.6 A), b = 5.66 nm (56.6 A), c = 13.79 nm (13.9 A) and beta = 99.7 degrees. Some unusual effects of poly(ethylene glycol)on protein crystals were noted and are discussed.
摘要
小鼠骨髓瘤蛋白M313的Fv片段从聚乙二醇溶液中结晶，形成单斜晶体，空间群为C2，晶胞参数a = 5.96纳米（59.6埃），b = 5.66纳米（56.6埃），c = 13.79纳米（139埃），β = 99.7度。注意到聚乙二醇对蛋白质晶体有一些不寻常的影响并进行了讨论。
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本文引用的文献
1
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
2
Crystallization with hapten of the Fab' fragment from a mouse IgA myeloma protein with antidinitrophenyl activity.具有抗二硝基苯基活性的小鼠IgA骨髓瘤蛋白Fab'片段与半抗原的结晶。
J Biol Chem. 1971 Oct 25;246(20):6272-5.
3
Amino acid sequence of the light chain of a mouse myeloma protein (MOPC-315).一种小鼠骨髓瘤蛋白（MOPC - 315）轻链的氨基酸序列。
Biochemistry. 1973 Dec 18;12(26):5400-16.
4
An active antibody fragment (Fv) composed of the variable portions of heavy and light chains.一种由重链和轻链可变部分组成的活性抗体片段（Fv）。
Biochemistry. 1973 Mar 13;12(6):1130-5. doi: 10.1021/bi00730a018.
5
Localization of antibody-combining sites within the variable portions of heavy and light chains.抗体结合位点在重链和轻链可变区的定位。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2659-62. doi: 10.1073/pnas.69.9.2659.
6
Amino-acid sequence of the variable region of the heavy (alpha) chain of a mouse myeloma protein with anti-hapten activity.具有抗半抗原活性的小鼠骨髓瘤蛋白重链（α链）可变区的氨基酸序列。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1123-7. doi: 10.1073/pnas.71.4.1123.
7
Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptide.结合硝基苯基配体的小鼠骨髓瘤蛋白的亲和标记。标记动力学及标记肽段的分离
Biochemistry. 1970 Mar 3;9(5):1267-78. doi: 10.1021/bi00807a031.
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9
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J Biol Chem. 1976 Oct 25;251(20):6300-3.
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