The Marine Biological Association, The Laboratory, Citadel Hill, Plymouth PL1 2PB, UK.
Environ Pollut. 2010 May;158(5):1702-9. doi: 10.1016/j.envpol.2009.11.024.
The effect of high hydrostatic pressures on the ecotoxicological profile of pollutants is an unexplored research area. Using Pomatoceros lamarcki as a surrogate organism for this eco-barotoxicological study, it was found that in a 48 h larval bioassay with water accommodated fractions (WAF) of crude oil of up to 15.1 mg L(-1) (total hydrocarbon content) and hydrostatic pressures up to 300 bar (3000 m), an additive response was found (p < 0.001) rather than any synergism (p = 0.881). Comprehensive cytogenetic analysis of 6-h (15 degrees C) embryos exposed to WAF (0.19 mg L(-1)) at 100 bar showed no effects on mitotic fidelity or cell division rate over the 1 bar treatment. However, embryo's treated with the clastogen mitomycin-c at 100 bar exhibited a significant increase in mitotic aberrations over 1 bar treated as was the case with hypo/hypersaline treatments (p < 0.05). Conversely, an increase in hydrostatic pressure actually reduced the effects of spindle inhibition by the aneugen colchicine (p < 0.05).
高静水压对污染物生态毒理学特性的影响是一个尚未开发的研究领域。本生态毒理学研究采用 Pomatoceros lamarcki 作为替代生物，结果发现，在为期 48 小时的幼虫生物测定中，当水可分配分数（WAF）中原油含量高达 15.1mg/L（总碳氢化合物含量），静水压高达 300 巴（3000 米）时，观察到了一种附加反应（p<0.001），而不是任何协同作用（p=0.881）。对在 100 巴下暴露于 WAF（0.19mg/L）的 6 小时（15°C）胚胎进行综合细胞遗传学分析表明，在 1 巴处理条件下，有丝分裂保真度或细胞分裂率没有受到影响。然而，与 1 巴处理相比，在 100 巴下用致裂剂丝裂霉素 C 处理的胚胎，有丝分裂异常显著增加，高盐/低盐处理也是如此（p<0.05）。相反，静水压力的增加实际上降低了纺锤体抑制剂秋水仙素的抑制效果（p<0.05）。
