Department of Biological and Environmental Sciences, General Microbiology, University of Helsinki, P.O. Box 56, (Biocenter 1C), 00014 Helsinki, Finland.
Environ Pollut. 2010 May;158(5):1680-8. doi: 10.1016/j.envpol.2009.11.026. Epub 2009 Dec 21.
To reveal the degradation capacity of bacteria in PAH polluted soil and rhizosphere we combined bacterial extradiol ring-cleavage dioxygenase and 16S rRNA analysis in Betula pubescens rhizoremediation. Characterisation of the functional bacterial community by RFLP revealed novel environmental dioxygenases, and their putative hosts were studied by 16S rRNA amplification. Plant rhizosphere and PAH amendment effects were detected by the RFLP/T-RFLP analysis. Functional species richness increased in the birch rhizosphere and PAH amendment impacted the compositional diversity of the dioxygenases and the structural 16S rRNA community. A shift from an Acidobacteria and Verrucomicrobia dominated to an Alpha- and Betaproteobacteria dominated community structure was detected in polluted soil. Clone sequence analysis indicated catabolic significance of Burkholderia in PAH polluted soil. These results advance our understanding of rhizoremediation and unveil the extent of uncharacterized functional bacteria to benefit bioremediation by facilitating the development of the molecular tool box to monitor bacterial populations in biodegradation.
为了揭示受多环芳烃（PAH）污染土壤和根际中细菌的降解能力，我们结合桦树根际修复中的细菌外二醇环裂解双加氧酶和 16S rRNA 分析。通过 RFLP 对功能细菌群落的特征分析揭示了新的环境双加氧酶，通过 16S rRNA 扩增研究了它们的潜在宿主。通过 RFLP/T-RFLP 分析检测了植物根际和 PAH 添加剂的影响。桦树根际的功能物种丰富度增加，而 PAH 添加剂影响了双加氧酶和结构 16S rRNA 群落的组成多样性。在污染土壤中检测到从酸杆菌门和疣微菌门为主导转变为以 α-和β-变形菌门为主导的群落结构。克隆序列分析表明，在 PAH 污染土壤中，伯克霍尔德菌具有代谢作用。这些结果增进了我们对根际修复的理解，并揭示了未被描述的功能细菌的程度，通过促进生物修复分子工具包的发展来监测生物降解中的细菌种群，从而有利于生物修复。
