School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi'an 710032, China.
Biomaterials. 2010 Mar;31(8):2055-63. doi: 10.1016/j.biomaterials.2009.11.103. Epub 2009 Dec 21.
Titiania nanotubes have large potential in medical implant applications but their tissue compatibility is still controversial. Since the sterilization methods may impact the biocompatibility of titania nanotubes and be the source of the controversy, we investigate the influence of three commonly used sterilization methods, autoclaving, ultraviolet irradiation and ethanol immersion, on the cytocompatibility of titania nanotubes. Two titania nanostructures, namely nanonets with an average pore diameter of 25 nm and nanotubes with an average diameter of 80 nm, are used in this study. The results show that the sterilization methods significantly affect the cytocompatibility of these titania surfaces. UV and ethanol sterilization give rise to a higher surface free energy inducing higher initial cell adhesion and proliferation compared to autoclaving, whereas UV irradiation produces the best cell functions including adhesion, proliferation, as well as differentiation represented by related gene expressions. The cytocompatibility results obtained from the nanoscale surfaces are compared to those acquired from the polished surface demonstrating the significant effects. Our results suggest that the sterilization process plays an important role in the observed cytocompatibility of titania nanotubes and may be the reason for the controversial results so far. UV sterilization is found to be the best method from the viewpoint of surface contamination elimination.
标题：三种常用灭菌方法对氧化钛纳米管生物相容性的影响
摘要：氧化钛纳米管在医学植入物应用中有很大的潜力，但它们的组织相容性仍存在争议。由于灭菌方法可能会影响氧化钛纳米管的生物相容性，是争议的来源，我们研究了三种常用的灭菌方法，高压灭菌、紫外线照射和乙醇浸泡，对氧化钛纳米管细胞相容性的影响。本研究使用了两种氧化钛纳米结构，即平均孔径为 25nm 的纳米网和平均直径为 80nm 的纳米管。结果表明，灭菌方法显著影响这些氧化钛表面的细胞相容性。与高压灭菌相比，UV 和乙醇灭菌会导致更高的表面自由能，从而诱导更高的初始细胞黏附和增殖，而 UV 照射则产生了最佳的细胞功能，包括黏附、增殖以及相关基因表达所代表的分化。从纳米级表面获得的细胞相容性结果与从抛光表面获得的结果进行了比较，结果表明，灭菌过程在观察到的氧化钛纳米管的细胞相容性中起着重要作用，这可能是迄今为止产生争议的原因。从表面污染消除的角度来看，发现 UV 灭菌是最好的方法。
