Peng Lily, Eltgroth Matthew L, LaTempa Thomas J, Grimes Craig A, Desai Tejal A
Department of Bioengineering and Therapeutic Sciences, University of California, Box 2520, Byers Hall Rm 203C, San Francisco, CA 94158-2330, USA.
Biomaterials. 2009 Mar;30(7):1268-72. doi: 10.1016/j.biomaterials.2008.11.012. Epub 2008 Dec 11.
In this study we investigate the effects of nanotubular titanium oxide (TiO(2)) surfaces on vascular cells. EC and VSMC response to nanotubes was investigated through immunofluorescence staining, scanning electron microscopy, 5-ethynyl-2'-deoxyuridine proliferation assays, and prostaglandin I(2) (PGI(2)) enzyme immunoassays. We found that the nanotubular surface significantly enhances EC proliferation and secretion of PGI(2). The surface also results in a decrease in VSMC proliferation and increased expression of smooth muscle alpha-actin. These data suggest that engineered nanotopographical cues may influence both EC and VSMC behavior in a manner that may be useful for stent or other vascular applications.
在本研究中，我们调查了纳米管状二氧化钛（TiO₂）表面对血管细胞的影响。通过免疫荧光染色、扫描电子显微镜、5-乙炔基-2'-脱氧尿苷增殖测定以及前列腺素I₂（PGI₂）酶免疫测定来研究内皮细胞（EC）和平滑肌细胞（VSMC）对纳米管的反应。我们发现纳米管状表面显著增强了EC的增殖和PGI₂的分泌。该表面还导致VSMC增殖减少以及平滑肌α-肌动蛋白表达增加。这些数据表明，工程化的纳米拓扑线索可能以一种对支架或其他血管应用有用的方式影响EC和VSMC的行为。
