Institute of Pharmacy, Pharmacognosy, University of Innsbruck, Innrain 52, 6020 Innsbruck, Austria.
J Pharm Biomed Anal. 2010 Apr 6;51(5):1165-8. doi: 10.1016/j.jpba.2009.11.028. Epub 2009 Dec 1.
This manuscript reports on the first analytical procedure for the determination of flavonoids in Celtis australis. The capillary electrophoretic separation of 8 compounds, most of them flavone C-glycosides, was possible using a borax buffer with pH 9.0, which contained 25mM SDS as detergent and 7.5% of n-butanol as organic modifier. Method validation revealed that the developed assay is repeatable (sigma(rel)<or=4.0%), precise (inter-day sigma(rel)<or=6.7%, intra-day sigma(rel)</=3.9%), accurate (recovery rates from 96.8 to 102.3%), sensitive (LOD: 2.2-1.6microg/ml) and linear (R(2)>or=0.9996) within the tested concentration range. The quantitative analysis of several C. australis samples showed that isovitexin is the most abundant representative (0.06-0.09%), at a rather uniform content of total flavonoids of approx. 0.3% in all specimens.
这篇手稿报告了首个分析方法，用于测定朴树中的类黄酮。使用 pH 值为 9.0 的硼砂缓冲液，其中含有 25mM SDS 作为去污剂和 7.5%的正丁醇作为有机改性剂，实现了 8 种化合物（大多数为黄酮 C-糖苷）的毛细管电泳分离。方法验证表明，所开发的测定法具有可重复性（sigma(rel)<or=4.0%）、精确性（日间 sigma(rel)<or=6.7%，日内 sigma(rel)</=3.9%）、准确性（回收率为 96.8%至 102.3%）、灵敏度（LOD：2.2-1.6μg/ml）和线性（R(2)>or=0.9996），测试浓度范围内。对几种朴树样本的定量分析表明，异荭草苷是最丰富的代表（0.06-0.09%），所有标本中总类黄酮的含量约为 0.3%，相当均匀。
