Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran.
Am J Clin Pathol. 2010 Jan;133(1):102-6. doi: 10.1309/AJCPXLZPRHX9L0YG.
Vaccination of all newborns with bacille Calmette-Guérin (BCG) vaccine is a standard practice in developing countries. Disseminated mycobacterial infection in an immunocompromised child can be caused by BCG and other mycobacteria. A total of 21 patients with a histopathologic diagnosis of mycobacterial infection were studied in a period of 4 years. DNA was extracted from formalin-fixed, paraffin-embedded tissues. Real-time polymerase chain reaction was performed to determine the mycobacterial species. The overall sensitivity of the assay was 71.5%. The prevalence rates of BCG, Mycobacterium tuberculosis, and other mycobacteria in the positive results were 80% (12/15), 13% (2/15), and 7% (1/15), respectively.
所有新生儿接种卡介苗（BCG）疫苗是发展中国家的标准做法。免疫功能低下的儿童中，卡介苗和其他分枝杆菌可引起播散性分枝杆菌感染。在 4 年期间，对 21 例经组织病理学诊断为分枝杆菌感染的患者进行了研究。从福尔马林固定、石蜡包埋的组织中提取 DNA。实时聚合酶链反应用于确定分枝杆菌的种类。该检测方法的总灵敏度为 71.5%。阳性结果中 BCG、结核分枝杆菌和其他分枝杆菌的检出率分别为 80%（12/15）、13%（2/15）和 7%（1/15）。
