Molecular Immunology Unit, Institute of Child Health, University College London, Guilford Street, London, United Kingdom.
Integr Biol (Camb). 2009 Mar;1(3):260-6. doi: 10.1039/b819889f. Epub 2009 Jan 19.
Bio-electrospraying, a recently discovered, direct electric field driven cell engineering process, has been demonstrated to have no harmful effects on treated cells at a molecular level. Although several cell types from both immortalized and primary cultures have been assessed post-treatment as a function of time in comparison to controls, the protocol has yet to be applied on embryonic stem cells. This is most important if bio-electrosprays are to further their applicability, in particular with regard to tissue engineering and regenerative medicine, where embryonic stem cells play a fundamental role. In the study presented herein the chosen stem cells are mouse embryonic stem (ES) cells. Hence, these first examples where embryonic stem cells have been jetted by way of bio-electrosprays, demonstrate the cellular viability and the cell's pluripotency indistinguishable when comparing those post-treated cells with their respective controls.
生物电喷射是一种最近发现的、直接电场驱动的细胞工程过程，已被证明在分子水平上对处理过的细胞没有有害影响。尽管已经对几种来自永生化和原代培养的细胞类型进行了处理后与对照组进行了时间相关性的评估，但该方案尚未应用于胚胎干细胞。如果生物电喷射要进一步提高其适用性，特别是在组织工程和再生医学方面，胚胎干细胞发挥着至关重要的作用，那么这一点就显得尤为重要。在本研究中，选择的干细胞是小鼠胚胎干细胞(ES 细胞)。因此，这些胚胎干细胞首次通过生物电喷射喷射的例子表明，与各自的对照组相比，处理后的细胞的细胞活力和多能性没有区别。
