Lambert Jean-Philippe, Baetz Kristin, Figeys Daniel
Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
Mol Biosyst. 2010 Jan;6(1):30-7. doi: 10.1039/b907925b. Epub 2009 Sep 3.
To paraphrase Robert Burns's poem To a Mouse, the best laid schemes of DNA-protein complex purification often go awry. Chromatin with its heterogeneous and dynamic protein composition remains difficult to analyze. Still critical progress has been made in recent years in characterizing the interface between DNA and proteins due, in part, to significant advances in proteomic technologies. Proteomics has progressed to a point where affinity purification of soluble complexes and protein identification by mass spectrometry are routine. The new challenge for chromatin proteomics lies in studying proteins and protein complexes in their native environment, which is on chromatin. These novel types of data represent an additional layer of information that can be used to better characterize and understand cellular processes. This review will focus on the past contributions as well as on emerging mass spectrometry-based methodologies attempting to better define the complex relationship between proteins, protein complexes and DNA.
套用罗伯特·彭斯的诗作《致小鼠》的话来说，DNA-蛋白质复合物纯化的最佳计划常常会出岔子。染色质具有异质性和动态的蛋白质组成，仍然难以分析。不过，近年来在表征DNA与蛋白质之间的界面方面已经取得了关键进展，这在一定程度上归功于蛋白质组学技术的重大进步。蛋白质组学已经发展到这样一个阶段，即可溶性复合物的亲和纯化和通过质谱鉴定蛋白质已成为常规操作。染色质蛋白质组学的新挑战在于研究处于天然环境（即染色质上）的蛋白质和蛋白质复合物。这些新型数据代表了一层额外的信息，可用于更好地表征和理解细胞过程。本综述将聚焦于过去的贡献以及新兴的基于质谱的方法，这些方法试图更好地界定蛋白质、蛋白质复合物与DNA之间的复杂关系。
