Samuel Lunenfeld Research Institute at Mount Sinai Hospital, Toronto, ON, Canada.
Proteomics. 2012 May;12(10):1609-22. doi: 10.1002/pmic.201100547.
Our ability to study protein-protein interactions has grown by leaps and bounds in recent years, enabling numerous large-scale studies to be performed in a variety of organisms. Despite this success, some classes of proteins, including those bound to chromatin, remain difficult to characterize through proteomic approaches. Some of the problems faced by researchers studying chromatin-bound proteins include low complex solubility, heterogeneous sample composition, and numerous transient interactions, which can be further complicated by the presence of DNA itself. To tackle these issues, a number of innovative protocols have been developed to better study the various facets of chromatin biology. In this review, we will discuss novel approaches to study protein-DNA interactions as well as protein complexes affecting chromatin.
近年来，我们研究蛋白质-蛋白质相互作用的能力突飞猛进，使得在多种生物体中进行大量的大规模研究成为可能。尽管取得了这一成功，但仍有一些类别的蛋白质难以通过蛋白质组学方法进行表征，包括与染色质结合的蛋白质。研究与染色质结合的蛋白质的研究人员面临的一些问题包括低复合物溶解度、样品组成不均匀和许多瞬态相互作用，而 DNA 本身的存在会使这些问题进一步复杂化。为了解决这些问题，已经开发了许多创新的方案来更好地研究染色质生物学的各个方面。在这篇综述中，我们将讨论研究蛋白质-DNA 相互作用以及影响染色质的蛋白质复合物的新方法。
