牦牛（Bos grunniens）胃溶菌酶：分子克隆、表达及其抗菌活性。
Yak (Bos grunniens) stomach lysozyme: molecular cloning, expression and its antibacterial activities.
机构信息
Sichuan Key Laboratory of Conservation and Utilization of Animal Genetic Resources in Tibetan Plateau, Southwest University for Nationalities, Chengdu City, China.
出版信息
Anim Biotechnol. 2010;21(1):25-35. doi: 10.1080/10495390903339899.
The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432 bp open reading frame and encodes 143 amino acids (16.24 KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene PsiNS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni(2+)-column showed a molecular weight of 33.78 kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities.
克隆了牦牛胃溶菌酶的 cDNA。该克隆 cDNA 含有一个 432bp 的开放阅读框，编码 143 个氨基酸（16.24KDa），带有 18 个氨基酸的信号肽。进一步分析表明，其氨基酸序列与牛乳溶菌酶有许多共同特性。通过 RT-PCR 也在乳腺组织中检测到了该基因的表达。牦牛胃溶菌酶与 8 种牛溶菌酶的系统发育关系表明，牦牛酶与牛乳溶菌酶和假基因 PsiNS4 更为密切相关，而与牛胃溶菌酶关系较远。通过 Ni(2+)-柱纯化的重组牦牛溶菌酶的分子量为 33.78 kDa，对金黄色葡萄球菌具有溶菌活性，证明了其抗菌活性。
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