Department of Dental Biomaterials, School of Dentistry, Kyungpook National University, Jung-gu, Daegu 700-412, Korea.
J Biomed Mater Res B Appl Biomater. 2010 Feb;92(2):561-7. doi: 10.1002/jbm.b.31553.
This study evaluated the antibacterial effects of 4-acryloyloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butylborane (4-META/MMA-TBB) resin containing chlorhexidine (CHX) digluconate. The CHX was incorporated into the resins at varying concentrations from 0.0 (control) to 3.0%. The antibacterial effect of each resin against seven microorganisms was examined using agar diffusion tests. Growth inhibition of two streptococci was also tested in brain heart infusion (BHI) liquid broth containing each resin. Release of CHX was analyzed using HPLC, and enamel and dentin bond strengths of each resin were measured. In the agar diffusion tests, growth of all the microorganisms was inhibited by the resin specimens containing CHX at 1.0% or higher except for Enterococcus faecalis. There was greater sensitivity in Streptococcus mutans and Streptococcus sobrinus than in the other bacteria tested. Complete growth inhibition of these two streptococci was observed in BHI liquid broth containing 1.0-1.5% or greater CHX-incorporated resin. According to HPLC analysis, 1.0% and 1.5% CHX-containing resins released means of 3.63 and 8.59 microg/mL of CHX, respectively. Specimens with 0.5-1.5% and 0.5-2.0% CHX exhibited no significant reduction in enamel and dentin bond strengths, respectively, when compared to the control (p > 0.05). This in vitro study suggested that incorporation of 1.0-1.5% CHX digluconate into the 4-META resin is optimal in terms of antibacterial effects and bond strength to the tooth.
本研究评估了 4-丙烯酰氧乙基偏苯三酸酐/甲基丙烯酸甲酯-三正丁基硼（4-META/MMA-TBB）树脂中含氯己定（CHX）双葡萄糖酸盐的抗菌效果。将 CHX 以 0.0（对照）至 3.0%的不同浓度掺入树脂中。使用琼脂扩散试验检测每种树脂对七种微生物的抗菌效果。还在含有每种树脂的脑心浸液（BHI）液体肉汤中测试了两种链球菌的生长抑制情况。使用 HPLC 分析 CHX 的释放情况，并测量每种树脂的牙釉质和牙本质结合强度。在琼脂扩散试验中，除粪肠球菌外，所有含 CHX 浓度为 1.0%或更高的树脂标本均抑制了所有微生物的生长。变形链球菌和远缘链球菌比其他测试细菌更敏感。在含有 1.0-1.5%或更高浓度 CHX 掺入树脂的 BHI 液体肉汤中，观察到这两种链球菌的完全生长抑制。根据 HPLC 分析，1.0%和 1.5%含 CHX 的树脂分别释放 3.63 和 8.59μg/mL 的 CHX。与对照相比（p>0.05），含有 0.5-1.5%和 0.5-2.0% CHX 的标本在牙釉质和牙本质结合强度方面没有明显降低。这项体外研究表明，4-META 树脂中掺入 1.0-1.5%的 CHX 双葡萄糖酸盐在抗菌效果和与牙齿的结合强度方面是最佳的。
