Ata Secil Ozkan, Akay Canan, Mumcu Emre, Erdonmez Demet
Department of Prosthodontics, Faculty of Dentistry, Osmangazi University, Eskisehir, Turkey (Corresponding author).
Advanced Material Technologies Application and Research Center, Osmangazi University, Eskisehir, Turkey.
Acta Stomatol Croat. 2023 Sep;57(3):238-247. doi: 10.15644/asc57/3/4.
The surface of provisional restorations applied before conventional or implant- supported fixed restorations may cause bacterial or fungal biofilm formation. The aim of this study was to evaluate the antimicrobial activity of acrylic resins used in provisional restorations modified with chlorhexidine diacetate.
120 cylindrical, auto-polymerized resin samples modified with chlorhexidine diacetate were prepared at concentrations of 0 (control), 1, 3, 5 wt %. The antimicrobial activity was examined against , , and using Crystal Violet quantification, MTT assay, and Scanning Electron Microscopy. Data were analyzed by ANOVA and paired sample t-tests (α=0.05).
The addition of chlorhexidine diacetate influenced the growth rate and metabolic activity of microorganisms. The antimicrobial effect against and statistically increased with the percentage of chlorhexidine diacetate. bacteria were less affected by chlorhexidine diacetate compared to other pathogens.
It has been shown that the effectiveness of CHDA in inhibiting the proliferation of microorganisms correlated positively with increasing concentration levels. More research is needed to confirm the impact of different chlorhexidine concentrations on the mechanical properties, clinical efficacy, and antimicrobial properties of CDHA.
在传统或种植体支持的固定修复体应用之前所使用的临时修复体表面可能会导致细菌或真菌生物膜形成。本研究的目的是评估用双醋酸氯己定改性的用于临时修复体的丙烯酸树脂的抗菌活性。
制备120个用双醋酸氯己定改性的圆柱形自凝树脂样品，浓度分别为0（对照）、1、3、5 wt%。使用结晶紫定量法、MTT法和扫描电子显微镜对 、 和 进行抗菌活性检测。数据采用方差分析和配对样本t检验进行分析（α=0.05）。
双醋酸氯己定的添加影响了微生物的生长速率和代谢活性。对 和 的抗菌效果随双醋酸氯己定百分比的增加而在统计学上增加。与其他病原体相比， 细菌受双醋酸氯己定的影响较小。
已表明CHDA在抑制微生物增殖方面的有效性与浓度水平的增加呈正相关。需要更多研究来证实不同氯己定浓度对CDHA的机械性能、临床疗效和抗菌性能的影响。
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