内生真菌枝顶孢霉合成抗菌银纳米粒子。

Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus.

机构信息

Mycopathology & Microbial Technology Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.

出版信息

Nanomedicine (Lond). 2010 Jan;5(1):33-40. doi: 10.2217/nnm.09.77.

DOI:10.2217/nnm.09.77

PMID:20025462

Abstract

AIM

To induce the biosynthesis of silver nanoparticles (AgNPs) using Aspergillus clavatus and evaluate their antimicrobial potential.

MATERIALS & METHODS: Aspergillus clavatus (AzS-275), an endophytic fungus isolated from sterilized stem tissues of Azadirachta indica A. Juss., was challenged with 1 mM AgNO(3) solution. The characterization of the AgNPs was carried out by x-ray diffraction spectrometry, transmission-electron microscopy and atomic force microscopy.

RESULTS & DISCUSSION: The synthesized AgNPs were found to be extracellular, polydispersed spherical or hexagonal particles ranging from 10 to 25 nm in size. Antimicrobial activity was performed using a disc-diffusion method against Candida albicans, Pseudomonas fluorescens and Escherichia coli. The results showed an average minimum inhibitory concentration of 5.83 microg ml(-1) and minimum fungicidal concentration of 9.7 microg ml(-1) against C. albicans.

CONCLUSIONS

AgNPs can be mycosynthesized extracellularly using A. clavatus as the fungal system, which is highly advantageous over chemical synthesis not only because it can be synthesized on a large scale, but because of the ease of downstream processing and its biomedical application in antimicrobial activity.

摘要

目的

利用青霉（Aspergillus clavatus）诱导合成银纳米粒子（AgNPs），并评估其抗菌潜力。

材料与方法

从消毒的印楝（Azadirachta indica A. Juss.）茎组织中分离出内生真菌青霉（AzS-275），用 1mM AgNO（3）溶液对其进行挑战。采用 X 射线衍射光谱法、透射电子显微镜和原子力显微镜对 AgNPs 进行了表征。

结果与讨论

所合成的 AgNPs 为细胞外、多分散的球形或六方纳米粒子，尺寸为 10-25nm。采用圆盘扩散法对白色念珠菌（Candida albicans）、荧光假单胞菌（Pseudomonas fluorescens）和大肠杆菌（Escherichia coli）进行了抗菌活性测试。结果表明，AgNPs 对白色念珠菌的平均最小抑菌浓度为 5.83μgml（-1），最小杀菌浓度为 9.7μgml（-1）。