一种抗Brg1大鼠单克隆抗体的制备。

Production of a rat monoclonal antibody against Brg1.

作者信息

Ohkawa Yasuyuki, Harada Akihito, Nakamura Mako, Yoshimura Saori, Tachibana Taro

机构信息

SSP Stem Cell Unit, Department of Epigenetics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Hybridoma (Larchmt). 2009 Dec;28(6):463-6. doi: 10.1089/hyb.2009.0041.

DOI:10.1089/hyb.2009.0041

PMID:20025509

Abstract

Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

摘要

Brm相关基因1（Brg1）是具有ATP酶活性的SWI/SNF染色质重塑酶复合物的催化亚基。该复合物通过利用ATP水解产生的能量促进染色质重塑以实现基因表达。众所周知，SWI/SNF染色质重塑酶复合物对于细胞分化、细胞周期调控和胚胎发生至关重要。在此，我们报告通过大鼠髂内侧淋巴结法建立了用于生产抗Brg1亚基抗体的杂交瘤细胞系。免疫印迹分析表明我们的抗体能够特异性识别Brg1。免疫细胞化学显示Brg1位于C2C12成肌细胞核的常染色质中。这些数据表明该抗体可用于分析细胞中Brg1蛋白的分子功能。