针对Brm的大鼠单克隆抗体的产生。

Generation of a rat monoclonal antibody specific for Brm.

作者信息

Okada Seiji, Harada Akihito, Saiwai Hirokazu, Nakamura Mako, Ohkawa Yasuyuki

机构信息

SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Hybridoma (Larchmt). 2009 Dec;28(6):455-8. doi: 10.1089/hyb.2009.0044.

DOI:10.1089/hyb.2009.0044

PMID:20025507

Abstract

Brm is a subunit of the SWI/SNF complex that has a ATPase activity. It is well known that the complex plays a major role in cell processes, such as proliferation, differentiation, and DNA repair of cells. Here we report the production of monoclonal antibody (1H7A10) against Brm by rat medial iliac lymph node method. Immunoblot analysis with the antibody revealed the specific recognition of Brm and increase of Brm protein level in skeletal muscle differentiation. Immunocytochemistry analysis shows nuclear localization in myoblast C2C12 and involvement of transcription in the late stages of differentiation.

摘要

Brm是具有ATP酶活性的SWI/SNF复合物的一个亚基。众所周知，该复合物在细胞增殖、分化和DNA修复等细胞过程中发挥着重要作用。在此，我们报告通过大鼠髂内侧淋巴结法制备抗Brm的单克隆抗体（1H7A10）。用该抗体进行免疫印迹分析显示，其能特异性识别Brm，并在骨骼肌分化过程中使Brm蛋白水平升高。免疫细胞化学分析表明，其在成肌细胞C2C12中定位于细胞核，且参与分化后期的转录过程。