Yoshimura Saori, Yoshimi Tomohiko, Ohkawa Yasuyuki, Azuma Masayuki, Tachibana Taro
Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Hybridoma (Larchmt). 2010 Feb;29(1):63-6. doi: 10.1089/hyb.2009.0069.
CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.
CHD5（染色质结构域/解旋酶/DNA结合蛋白5）是染色质重塑Swi/Snf蛋白CHD亚家族的成员，最近被确定为多种人类癌症中的一种肿瘤抑制因子。我们在此报告通过大鼠髂内侧淋巴结法建立了一种用于生产抗CHD5单克隆抗体的杂交瘤细胞系。免疫印迹分析表明，该抗体MAb 5A10能特异性识别内源性CHD5。在用该抗体进行免疫染色时，观察到核染色模式。这种单克隆抗体将有助于在各种细胞和组织中进行免疫印迹和免疫定位实验，以及对该蛋白的生物学功能和细胞动力学进行进一步研究。
