Biolab S.L., Colmenar Viejo, Madrid, Spain.
Acta Trop. 2010 Apr;114(1):22-30. doi: 10.1016/j.actatropica.2009.12.002. Epub 2009 Dec 16.
In the present paper, the acute and subchronical inflammatory processes of the vaginal epithelial were studied in mice experimentally infected with two Trichomonas vaginalis strains of different pathogenicity, by means of histological and immunological methods. There was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. The proliferation of the vaginal epithelium favors the settlement and persistence of the parasitic infection. All of the findings corresponded with signs of a systemic disease being observed in the animals, including significant weight loss and also intestinal invasion. The entire inflammatory process has been corroborated by studies of adhesion molecules such as E-Selectin, VCAM-1 and PECAM-1. A correlation between the time of appearance and the perseverance of the inflammatory process with E-Selectin and VCAM-1 expression was observed, but not with PECAM-1. The strain with a higher pathogenicity was able to invade deep vaginal tissues and thus, parasites could not be detected by vaginal washings. This may be an important cause of diagnosis and treatment failure. Also, by the different localization of trichomonads, it appeared that the battle between host and parasite took place in different areas dependent upon the characteristics of the strain.
本研究采用组织学和免疫学方法，对经两种不同致病性阴道毛滴虫株感染的小鼠阴道上皮的急性和亚慢性炎症过程进行了研究。固有黏膜下血管化增加导致的分层上皮层和水肿增加，以及白细胞浸润导致炎症反应。阴道上皮的增生有利于寄生虫感染的定居和持续存在。所有发现都与观察到的动物全身性疾病的迹象相对应，包括明显的体重减轻和肠道侵袭。黏附分子（如 E-选择素、VCAM-1 和 PECAM-1）的研究证实了整个炎症过程。观察到 E-选择素和 VCAM-1 表达与炎症过程的出现和持续时间之间存在相关性，但与 PECAM-1 无关。致病性更高的菌株能够侵入阴道深部组织，因此，阴道冲洗无法检测到寄生虫。这可能是诊断和治疗失败的一个重要原因。此外，通过滴虫的不同定位，似乎宿主和寄生虫之间的斗争发生在不同的区域，这取决于菌株的特征。
