College of Life Sciences and Biotechnology, Dalian Fisheries University, Dalian 116023, China.
Comp Biochem Physiol B Biochem Mol Biol. 2010 Mar;155(3):327-30. doi: 10.1016/j.cbpb.2009.12.001. Epub 2009 Dec 22.
Myostatin (MSTN) is a member of the transforming growth factor-beta superfamily which negatively regulates growth of muscle tissue. In this study, 103 cultivated Chlamys farreri individuals were screened for polymorphisms in the MSTN gene using PCR-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods. Two mutations were found: A/G at position 327 in exon 2, which caused an amino acid change from Thr to Ala (Thr305Ala), and C/T at position 289 in exon 3, which caused an amino acid change from Cys to Arg (Cys422Arg). One way ANOVA of the SNPs and growth traits showed that genotype GG of primer M5 had significantly higher body mass, soft-tissue mass, adductor muscle mass, shell length, shell height, absolute growth rate of shell height and body mass than those of genotype AG and AA (P<0.05). Genotype frequencies of genotype AA, AG and GG were 68.94%, 27.18% and 3.88%, respectively. The results present evidence that the C. farreri MSTN gene may be selected as a candidate gene for these growth traits.
肌肉生长抑制素（MSTN）是转化生长因子-β超家族的一员，它负调控肌肉组织的生长。本研究采用 PCR-单链构象多态性（PCR-SSCP）和 DNA 测序方法对 103 个养殖栉孔扇贝个体的 MSTN 基因进行多态性筛选。发现了两个突变：第 2 外显子 327 位的 A/G，导致氨基酸从苏氨酸变为丙氨酸（Thr305Ala）；第 3 外显子 289 位的 C/T，导致氨基酸从半胱氨酸变为精氨酸（Cys422Arg）。单因素方差分析 SNP 和生长性状的结果表明，引物 M5 的 GG 基因型的体重、软组织质量、扇贝肌质量、壳长、壳高、壳高和体重的绝对增长率显著高于 AG 和 AA 基因型（P<0.05）。AA、AG 和 GG 基因型的频率分别为 68.94%、27.18%和 3.88%。结果表明，栉孔扇贝 MSTN 基因可能被选为这些生长性状的候选基因。
