肌抑素基因外显子 1 突变对边鸡生长性状的影响。

Effect of an exon 1 mutation in the myostatin gene on the growth traits of the Bian chicken.

机构信息

College of Animal Science and Technology, Yangzhou University, China.

出版信息

Anim Genet. 2012 Aug;43(4):458-9. doi: 10.1111/j.1365-2052.2011.02274.x. Epub 2011 Oct 18.

DOI:10.1111/j.1365-2052.2011.02274.x

Abstract

Myostatin (MSTN), or growth and differentiation factor 8 (GDF8), is a member of the transforming growth factor (TGF)-β superfamily. This family functions as a negative regulator of skeletal muscle development and growth in mammals. Single-nucleotide polymorphisms in exon 1 of the Bian chicken myostatin gene were detected by polymerase chain reaction-restriction fragment length polymorphism. A mutation (c.234G>A) in exon 1 was found. Female Bian chickens of genotypes AA and GA had significantly higher body weights than those of genotype GG (P < 0.05 or P < 0.01) from 6 to 18 weeks of age. These results suggested that the mutation c.234G>A in exon 1 could be used as a genetic marker for Bian chicken growth traits.

摘要

肌肉生长抑制素（MSTN），也称为生长分化因子 8（GDF8），是转化生长因子（TGF）-β超家族的成员。该家族在哺乳动物中作为骨骼肌肉发育和生长的负调控因子发挥作用。通过聚合酶链反应-限制性片段长度多态性检测到比安鸡肌肉生长抑制素基因外显子 1 中的单核苷酸多态性。在外显子 1 中发现了一个突变（c.234G>A）。6 至 18 周龄时，基因型为 AA 和 GA 的雌性比安鸡的体重明显高于基因型为 GG 的比安鸡（P<0.05 或 P<0.01）。这些结果表明，外显子 1 中的突变 c.234G>A 可以作为比安鸡生长性状的遗传标记。