Laboratory of Natural Substances Chemistry, Faculty of Science, Sfax, Tunisia.
Mem Inst Oswaldo Cruz. 2009 Nov;104(7):1035-8. doi: 10.1590/s0074-02762009000700017.
The molluscicidal activity of Hammada scoparia leaf extracts and the principal alkaloids isolated from them (carnegine and N-methylisosalsoline) were tested against the mollusc gastropod, Galba truncatula, the intermediate host of Fasciola hepatica in Tunisia. The results indicated that the molluscicidal activity was correlated with the presence of alkaloids. A significant molluscicidal value, according to the World Health Organization, was found with the methanol extract (LC50 = 28.93 ppm). Further fractionation of the methanolic extract led to the isolation of two principal alkaloids: carnegine and N-methylisosalsoline. These alkaloids are isoquinolines that have not previously been characterised for their molluscicidal activity. The N-methylisosalsoline possesses the highest molluscicidal activity (LC50 = 0.47 microM against G. truncatula).
对来自哈马达·斯科皮亚（Hammada scoparia）的叶提取物及其主要生物碱（卡内基宁和 N-甲基异萨尔索林）进行了测试，以评估其对在突尼斯作为肝片吸虫中间宿主的圆口螺门螺类腹足纲动物（Galba truncatula）的灭螺活性。结果表明，灭螺活性与生物碱的存在有关。甲醇提取物的灭螺活性具有显著的世界卫生组织价值（LC50=28.93ppm）。进一步对甲醇提取物进行分段，分离出两种主要生物碱：卡内基宁和 N-甲基异萨尔索林。这些生物碱是异喹啉类化合物，其灭螺活性以前尚未得到描述。N-甲基异萨尔索林具有最高的灭螺活性（对 G. truncatula 的 LC50=0.47μM）。
