苦瓜叶提取物及其主要葫芦素对肝片吸虫中间宿主圆口铜腹螺的杀软体动物和杀幼虫活性研究。
A study of the molluscicidal and larvicidal activities of Citrullus colocynthis (L.) leaf extract and its main cucurbitacins against the mollusc Galba truncatula, intermediate host of Fasciola hepatica.
机构信息
Laboratory of Chemistry of Natural Substances, Faculty of Sciences, University of Sfax, Sfax, Tunisia.
Fungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Sfax, Tunisia.
出版信息
Pest Manag Sci. 2017 Jul;73(7):1473-1477. doi: 10.1002/ps.4479. Epub 2016 Dec 28.
BACKGROUND
The molluscicidal and larvicidal activities of the medicinal plant Citrullus colocynthis leaf extracts and its main cucurbitacins were tested against the mollusc gastropod Galba truncatula, the intermediate host of Fasciola hepatica.
RESULTS
Our findings proved for the first time that the molluscicidal activity was correlated with the presence of terpenoids. A significant molluscicidal value was found in the ethyl acetate extract (LC50 = 12.6 mg L ). Further fractionation of this extract led to the isolation of two main compounds identified to cucurbitacin E 1 and 2-O-β-d-glucocucurbitacin E 2. Their molluscicidal activities were also investigated and they possessed close activities with LC = 9.55 and 10.61 mg L for compounds 2 and 1, respectively.
CONCLUSION
The ethyl acetate extract and both pure compounds proved the highest larvicidal activities, with a deterioration rate exceeding 89.2% (89.2-100%) and with no toxic effects against associated fauna. © 2016 Society of Chemical Industry.
背景
药用植物西瓜叶提取物及其主要葫芦素对中间宿主肝片吸虫的软体动物门腹足纲动物具有杀软体动物和杀幼虫活性。
结果
我们的研究结果首次证明，杀软体动物活性与萜类化合物的存在有关。在乙酸乙酯提取物中发现了显著的杀软体动物活性（LC50 = 12.6 mg/L）。进一步对该提取物进行分离，得到了两种主要化合物，鉴定为葫芦素 E1 和 2-O-β-d-葡萄糖葫芦素 E2。还研究了它们的杀幼虫活性，化合物 2 和 1 的 LC 分别为 9.55 和 10.61 mg/L，活性相近。
结论
乙酸乙酯提取物和两种纯化合物均表现出最高的杀幼虫活性，恶化率超过 89.2%（89.2-100%），对相关动物群无毒性作用。© 2016 化学工业协会。
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