Laboratoire d'Ecophysiologie Animale, Faculté des Sciences de Sfax, Sfax, Tunisia.
J Physiol Biochem. 2013 Jun;69(2):227-37. doi: 10.1007/s13105-012-0206-7. Epub 2012 Aug 15.
The present work was aimed at studying the antioxidative activity and hepatoprotective effects of methanolic extract (ME) of Hammada scoparia leaves against ethanol-induced liver injury in male rats. The animals were treated daily with 35 % ethanol solution (4 g kg(-1) day(-1)) during 4 weeks. This treatment led to an increase in the lipid peroxidation, a decrease in antioxidative enzymes (catalase, superoxide dismutase, and glutathione peroxidase) in liver, and a considerable increase in the serum levels of aspartate and alanine aminotransferase and alkaline phospahatase. However, treatment with ME protects efficiently the hepatic function of alcoholic rats by the considerable decrease in aminotransferase contents in serum of ethanol-treated rats. The glycogen synthase kinase-3 β was inhibited after ME administration, which leads to an enhancement of glutathione peroxidase activity in the liver and a decrease in lipid peroxidation rate by 76 %. These biochemical changes were consistent with histopathological observations, suggesting marked hepatoprotective effect of ME. These results strongly suggest that treatment with methanolic extract normalizes various biochemical parameters and protects the liver against ethanol induced oxidative damage in rats.
本研究旨在探讨沙冬青叶甲醇提取物（ME）对乙醇诱导的雄性大鼠肝损伤的抗氧化活性和肝保护作用。动物在 4 周内每天用 35%乙醇溶液（4 g/kg 体重/天）处理。这种处理导致肝脏脂质过氧化增加，抗氧化酶（过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）减少，血清天门冬氨酸和丙氨酸氨基转移酶及碱性磷酸酶水平显著升高。然而，ME 处理可通过显著降低乙醇处理大鼠血清中转氨酶含量，有效地保护酒精性大鼠的肝功能。ME 给药后，糖原合成酶激酶-3β被抑制，导致肝脏谷胱甘肽过氧化物酶活性增强，脂质过氧化率降低 76%。这些生化变化与组织病理学观察结果一致，提示 ME 具有明显的肝保护作用。这些结果强烈表明，用甲醇提取物治疗可使各种生化参数正常化，并保护肝脏免受乙醇诱导的氧化损伤。
