Cátedra de Química Agrícola, ETSI Agrónomos de Albacete, Universidad de Castilla-La Mancha, 02071 Albacete, Spain.
J Agric Food Chem. 2010 Jan 27;58(2):1305-12. doi: 10.1021/jf903336t.
In this paper, 345 saffron samples were analyzed from different countries to study their picrocrocin content using different analytical techniques. The E1cm 1% of 257 nm results from all samples are inflated in comparison by the high-performance liquid chromatography (HPLC) data, because of the interferences with the crocetin ester pool and especially with those with a lower trans/cis relation. A picrocrocin range update is proposed for International Organization for Standardization (ISO) 3632 normative because category III should be incremented up to 50 units, while category II should be incremented up to 60 units. More accurate data are achieved when the deltaEpic measurement is carried out. Consequently, improvements to the ISO method are suggested. Fourier transform (FT)-near-infrared spectrometry analysis has also been carried out, showing excellent results from the calibration with HPLC data. This spectrophotometric technique could be used by saffron enterprises to obtain quick and more accurate data for picrocrocin determination.
本文对来自不同国家的 345 份藏红花样本进行了分析，使用不同的分析技术研究其吡咯里西啶生物碱含量。由于藏红花酯库，特别是反式/顺式比例较低的藏红花酯库的干扰，所有样本的 257nm 处的 E1cm 1%值都被高效液相色谱（HPLC）数据所夸大。建议对国际标准化组织（ISO）3632 标准中的吡咯里西啶生物碱范围进行更新，因为 III 类应增加到 50 个单位，而 II 类应增加到 60 个单位。当进行 deltaEpic 测量时，会得到更准确的数据。因此，建议对 ISO 方法进行改进。还进行了傅里叶变换（FT）-近红外光谱分析，结果表明与 HPLC 数据的校准效果非常好。这种分光光度技术可被藏红花企业用于获得快速、更准确的吡咯里西啶生物碱含量数据。
