Cátedra de Química Agrícola, E.T.S.I. Agrónomos, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, Spain.
Instituto Técnico Agronómico Provincial (ITAP), S.A. Avda. Gregorio Arcos, s/n., 02006 Albacete, Spain.
Food Chem. 2017 Apr 15;221:838-843. doi: 10.1016/j.foodchem.2016.11.089. Epub 2016 Nov 21.
The aim of this work was a comparison of the ISO 3632 (2011) method and an HPLC-DAD method for safranal quantity determination in saffron. Samples from different origins were analysed by UV-vis according to ISO 3632 (2011) and by HPLC-DAD. Both methods were compared, and there was no correlation between the safranal content obtained by UV-vis and HPLC-DAD. An over-estimation in the UV-vis experiment was observed, which was related to the cis-crocetin esters content, as well as other compounds. The results demonstrated that there was no relationship between ISO quality categories and safranal content using HPLC-DAD. Therefore, HPLC-DAD might be preferable to UV-vis for determining the safranal content and the classification of saffron for commercial purposes. In addition, HPLC-DAD was adequate for determining the three foremost parameters that define the quality of saffron (crocetin esters, picrocrocin and safranal); therefore, this approach could be included in the ISO 3632 method (2011).
本工作旨在比较 ISO 3632（2011）方法和 HPLC-DAD 方法测定藏红花中藏红花醛的含量。根据 ISO 3632（2011），用 UV-vis 和 HPLC-DAD 分析了来自不同来源的样品。对两种方法进行了比较，UV-vis 和 HPLC-DAD 得到的藏红花醛含量之间没有相关性。在 UV-vis 实验中观察到了过高的估计值，这与顺式藏红花酸酯的含量以及其他化合物有关。结果表明，使用 HPLC-DAD 时，ISO 质量类别与藏红花醛含量之间没有关系。因此，HPLC-DAD 可能优于 UV-vis，可用于确定藏红花的藏红花醛含量和商业分类。此外，HPLC-DAD 足以测定定义藏红花质量的三个最重要参数（藏红花酸酯、苦藏花素和藏红花醛）；因此，这种方法可以包含在 ISO 3632（2011）方法中。
