Saini A, Dua A, Mohindra V
Aquatic Biology Laboratory, Department of Zoology, Guru Nanak Dev University, Amritsar-143005 Pb, India.
Genetika. 2010 Aug;46(8):1102-7.
The Giant river catfish, Sperata seenghala (Sykes) is commercially very important fish species of South Asia. Genetic variability between its populations collected from two rivers i.e. river Sutlej and river Beas of Indus river system in India were examined using randomly amplified polymorphic DNA analysis. Total 38 fish samples were collected from river Sutlej whereas 46 fish samples were collected from river Beas. Total 40 primers were screened, out of these 7 were selected for studying polymorphism which produced a total of 64 RAPD loci in two populations. Percentage polymorphic loci calculated following 95% criterion was 89.06% for Beas population as compared to 95.31% for Sutlej population. Moderate level of genetic divergence (genetic distance of 0.0486) between both the populations suggests distinct population substructure of giant river catfish in both the rivers.
巨魾（Sperata seenghala (Sykes)）是南亚具有重要商业价值的鱼类物种。利用随机扩增多态性DNA分析，对从印度印度河系统的两条河流（即萨特莱杰河和比阿斯河）采集的该物种种群之间的遗传变异性进行了检测。从萨特莱杰河共采集了38个鱼类样本，从比阿斯河采集了46个鱼类样本。共筛选了40个引物，其中7个被选用于研究多态性，这7个引物在两个种群中总共产生了64个RAPD位点。按照95%的标准计算，比阿斯河种群的多态位点百分比为89.06%，而萨特莱杰河种群为95.31%。两个种群之间中等程度的遗传分化（遗传距离为0.0486）表明，这两条河流中的巨魾具有明显的种群亚结构。
