Universidade de São Paulo, Instituto de Química de São Carlos, Departamento de Química e Física Molecular, São Carlos, São Paulo, Brazil.
J Sep Sci. 2009 Nov;32(21):3681-91. doi: 10.1002/jssc.200900306.
A total of 25 sugarcane spirit extracts of six different Brazilian woods and oak, commonly used by cooperage industries for aging cachaça, were analyzed for the presence of 14 phenolic compounds (ellagic acid, gallic acid, vanillin, syringaldehyde, synapaldehyde, coniferaldehyde, vanillic acid, syringic acid, quercetin, trans-resveratrol, catechin, epicatechin, eugenol, and myricetin) and two coumarins (scopoletin and coumarin) by HPLC-DAD-fluorescence and HPLC-ESI-MS(n). Furthermore, an HPLC-DAD chromatographic fingerprint was build-up using chemometric analysis based on the chromatographic elution profiles of the extracts monitored at 280 nm. Major components identified and quantified in Brazilian wood extracts were coumarin, ellagic acid, and catechin, whereas oak extracts shown a major contribution of catechin, vanillic acid, and syringaldehyde. The main difference observed among oak and Brazilian woods remains in the concentration of coumarin, catechin, syringaldehyde, and coniferaldehyde. The chemometric analysis of the quantitative profile of the 14 phenolic compounds and two coumarins in the wood extracts provides a differentiation between the Brazilian wood and oak extracts. The chromatographic fingerprint treated by multivariate analysis revealed significant differences among Brazilian woods themselves and oak, clearly defining six groups of wood extracts: (i) oak extracts, (ii) jatobá extracts, (iii) cabreúva-parda extracts, (iv) amendoim extracts, (v) canela-sassafrás extracts and (vi) pequi extracts.
总共分析了六种不同巴西木材和橡木的 25 种甘蔗烈酒提取物，这些木材和橡木通常被制桶业用于陈酿甘蔗酒，以检测 14 种酚类化合物（鞣花酸、没食子酸、香草醛、丁香醛、丁香醛、松柏醛、香草酸、丁香酸、槲皮素、反式白藜芦醇、儿茶素、表儿茶素、丁香酚和杨梅素）和两种香豆素（东莨菪内酯和香豆素）的存在，方法是通过 HPLC-DAD-荧光和 HPLC-ESI-MS(n)。此外，还通过基于提取物色谱洗脱图谱的化学计量学分析，建立了 HPLC-DAD 色谱指纹图谱，该图谱在 280nm 处进行监测。在巴西木材提取物中鉴定和定量的主要成分是香豆素、鞣花酸和儿茶素，而橡木提取物则显示出儿茶素、香草酸和丁香醛的主要贡献。橡木和巴西木材之间观察到的主要区别在于香豆素、儿茶素、丁香醛和松柏醛的浓度。对木材提取物中 14 种酚类化合物和两种香豆素的定量图谱的化学计量学分析提供了巴西木材和橡木提取物之间的区别。通过多元分析处理的色谱指纹揭示了巴西木材本身和橡木之间的显著差异，清楚地定义了六组木材提取物：（i）橡木提取物，（ii）Jatobá 提取物，（iii）Cabreúva-parda 提取物，（iv）Amendoim 提取物，（v）Canela-sassafrás 提取物和（vi）Pequi 提取物。
