Puech J L, Rabier P, Bories-Azeau J, Sarni F, Moutounet M
Institut National de la Recherche Agronomique, Laboratoire des Polymères et des Techniques Physico-Chimiques, Montpellier, France.
J Assoc Off Anal Chem. 1990 Jul-Aug;73(4):498-501.
A method is described for determination of ellagitannins in ethanol-water extracts of oak wood and in distilled alcoholic beverages matured in oak barrels. It is based on the combined ellagic acid content according to ellagitannin structure. Hydrolysis was carried out in the presence of hydrochloric acid under reflux in a 100 degrees C oil bath for 3 h. Total ellagic acid was thus determined by liquid chromatography (LC), and the free ellagic acid content present in the ethanol-water media was subtracted, the difference being the combined ellagic acid content corresponding to ellagitannins. A 5 micron C18 column was used with detection at 254 nm. The method is specific for ellagitannins, which is an advantage over other analytical techniques for overall evaluation of these substances extracted from wood. Results for spirits distilled from wine, grain, and sugarcane were highly variable.
本文描述了一种用于测定橡木乙醇 - 水提取物以及在橡木桶中陈酿的蒸馏酒精饮料中鞣花单宁的方法。该方法基于根据鞣花单宁结构测定的总鞣花酸含量。水解在盐酸存在下于100℃油浴中回流3小时进行。通过液相色谱（LC）测定总鞣花酸，并减去乙醇 - 水介质中存在的游离鞣花酸含量，差值即为对应于鞣花单宁的总鞣花酸含量。使用5微米的C18柱，在254纳米处进行检测。该方法对鞣花单宁具有特异性，这是相对于用于全面评估从木材中提取的这些物质的其他分析技术的一个优势。从葡萄酒、谷物和甘蔗蒸馏得到的烈酒的结果差异很大。
