Hayashi Satoru, Nishiyama Miho, Suehisa Etsuji, Kashiwagi Hirokazu, Kurata Yoshiyuki, Tomiyama Yoshiaki
Department of Blood Transfusion, Osaka University Hospital, Suita 565-0879, Japan.
Rinsho Byori. 2009 Nov;57(11):1039-44.
Reticulated platelet (RP) is thought to be a useful marker for differential diagnosis and analysis of platelet kinetics in patients with thrombocytopenia. In this study, we compared two methods for the measurement of RP: flow cytometric (FCM) method and immature platelet fraction (IPF) method using automated hematology analyzer (XE-2000). There was a relatively good correlation between RP% measured by FCM method and IPF% measured by IPF method in patients with idiopathic thrombocytopenic purpura (ITP) (Y = 0.806X-0.050, r = 0.634, p < 0.001). We then measured RP% and IPF% in 61 patients with ITP and 27 patients with aplastic anemia (AA). For the differential diagnosis for ITP, the sensitivity (82%) and specificity (93%) of FCM method were better than those of IPF method (sensitivity 67% and specificity 63%). Our data demonstrate the significant difference between two methods by analyzing clinical samples in parallel.
网织血小板（RP）被认为是血小板减少症患者血小板动力学鉴别诊断和分析的有用标志物。在本研究中，我们比较了两种测量RP的方法：流式细胞术（FCM）法和使用自动血液分析仪（XE - 2000）的未成熟血小板分数（IPF）法。在特发性血小板减少性紫癜（ITP）患者中，通过FCM法测得的RP%与通过IPF法测得的IPF%之间存在相对良好的相关性（Y = 0.806X - 0.050，r = 0.634，p < 0.001）。然后我们测量了61例ITP患者和27例再生障碍性贫血（AA）患者的RP%和IPF%。对于ITP的鉴别诊断，FCM法的敏感性（82%）和特异性（93%）优于IPF法（敏感性67%，特异性63%）。我们的数据通过平行分析临床样本证明了两种方法之间的显著差异。
