Nishiyama Miho, Hayashi Satoru, Kabutomori Osamu, Yamanishi Hachirou, Suehisa Etsuji, Kurata Yoshiyuki, Kashiwagi Hirokazu, Tomiyama Yoshiaki
Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Japan.
Rinsho Byori. 2011 May;59(5):452-8.
Measurement of reticulated platelet percentage (RP%) is thought to be a useful marker for differential diagnosis and analysis of platelet kinetics in patients with thrombocytopenic disorders. Two methods are used to detect RP; flow cytometric method and immature platelet fraction (IPF) method using automated hematology analyzers. Although IPF% measured by the automated hematology analyzers is simple and convenient, we already reported that IPF% values were highly fluctuated in stored whole blood sample with EDTA-2K at 4 degrees C day by day. In this study we investigated the stability of IPF% in blood samples obtained from 11 patients with chronic immune thrombocytopenic purpura (ITP) and 19 healthy volunteers using the automated hematology analyzer, XE-5000 (Sysmex) under various storage conditions. EDTA-2K, 3.13% sodium citrate, acid-citrate dextrose solution (ACD), citrate-theophylline-adenosine-dipyridamole solution (CTAD), or sodium fluoride was used as an anticoagulant. When blood samples obtained from healthy subjects were stored at 4 degrees C, IPF% values markedly increased in a time-dependent manner by any anticoagulant examined. On the other hand, there was no significant or only slight difference in IPF% values at room temperature (RT) storage except sodium fluoride. However, in patients with ITP the elevated IPF% values fluctuated widely in EDTA-2K, sodium citrate and ACD-anticoagulated samples even at RT storage. In contrast, IPF% values in CTAD samples stored at RT were highly stable in all patients with ITP up to 4 day storage. These results suggest that the measurement of IPF% by XE-5000 provides quite stable data up to 4 day-storage in ITP patients as well as healthy subjects under CTAD-anticoagulation and RT storage conditions.
网织血小板百分比（RP%）的测定被认为是血小板减少性疾病患者鉴别诊断和血小板动力学分析的有用标志物。检测RP有两种方法；流式细胞术方法和使用自动血液分析仪的未成熟血小板分数（IPF）方法。尽管通过自动血液分析仪测量的IPF%简单方便，但我们已经报道，在4℃下用EDTA-2K保存的全血样本中，IPF%值每天都有很大波动。在本研究中，我们使用自动血液分析仪XE-5000（Sysmex）在各种储存条件下，研究了11例慢性免疫性血小板减少性紫癜（ITP）患者和19名健康志愿者的血液样本中IPF%的稳定性。使用EDTA-2K、3.13%柠檬酸钠、柠檬酸葡萄糖溶液（ACD）、柠檬酸-茶碱-腺苷-双嘧达莫溶液（CTAD）或氟化钠作为抗凝剂。当从健康受试者获得的血液样本在4℃下储存时，无论使用哪种检测的抗凝剂，IPF%值均以时间依赖性方式显著增加。另一方面，除氟化钠外，室温（RT）储存时IPF%值无显著差异或仅有轻微差异。然而，在ITP患者中，即使在RT储存时，EDTA-2K、柠檬酸钠和ACD抗凝样本中升高的IPF%值也有很大波动。相比之下，在RT储存的CTAD样本中，所有ITP患者的IPF%值在长达4天的储存期内都非常稳定。这些结果表明，在CTAD抗凝和RT储存条件下，XE-5000测量的IPF%在ITP患者以及健康受试者中长达4天的储存期内都能提供相当稳定的数据。
