Cannavo Isabelle, Ferrero-Vacher Corinne, Sudaka Isabelle, Aquaronne Danielle, Berthier Frédéric, Raynaud Sophie
Laboratoire d'hématologie, Hôpital de Cimiez, Nice.
Ann Biol Clin (Paris). 2010 Jul-Aug;68(4):415-20. doi: 10.1684/abc.2010.0449.
Reticulated platelets are young platelets containing mRNA. They reflect the rate of thrombopoesis. The aim of this study was to evaluate the reliability of the percentage of reticulated platelets (IPF%) as a diagnostic test for thrombocytopenia pathogenesis. IPF% was measured using XE 2100 Sysmex. An IPF% reference range in 52 healthy individuals was established as 1-4.5% with a median 2.2%. In all the 13 patients with idiopathic thrombocytopenic purpura IPF% was increased (median 11.8, range 5.3-54.3%). Only 7 out of 18 patients with disseminated intravascular coagulation had high IPF% (median 5.4%, range 2.9-14.1%). Surprisingly, IPF% was increased in 17 out of 22 patients with acute leukaemia (median 9.7%, range 0.9-41.9%). In CIVD, IPF% values correlated with the severity of the illness. Increased values in acute leukaemia could not be explained by non specific staining but by delayed maturation of reticulated platelets. A high IPF% does not substantiate hyperdestructive thrombocytopenia but a diagnosis of idiopathic thrombocytopenic purpura should be questioned if IPF% is not raised.
网织血小板是含有信使核糖核酸的年轻血小板。它们反映了血小板生成的速率。本研究的目的是评估网织血小板百分比（IPF%）作为血小板减少症发病机制诊断试验的可靠性。使用XE 2100 Sysmex测定IPF%。52名健康个体的IPF%参考范围确定为1 - 4.5%，中位数为2.2%。在所有13例特发性血小板减少性紫癜患者中，IPF%升高（中位数为11.8，范围为5.3 - 54.3%）。18例弥散性血管内凝血患者中只有7例IPF%升高（中位数为5.4%，范围为2.9 - 14.1%）。令人惊讶的是，22例急性白血病患者中有17例IPF%升高（中位数为9.7%，范围为0.9 - 41.9%）。在慢性炎症性血管病（CIVD）中，IPF%值与疾病严重程度相关。急性白血病中IPF%升高不能用非特异性染色解释，而是由于网织血小板成熟延迟。高IPF%不能证实血小板过度破坏，但如果IPF%未升高，则应质疑特发性血小板减少性紫癜的诊断。
