Medical Genetics, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria.
Reprod Biomed Online. 2009 Dec;19(6):838-46. doi: 10.1016/j.rbmo.2009.09.015.
Although dialysis and transplantation are widely applied therapies for renal failure, drawbacks such as morbidity, shortage of compatible organs and high cost are limiting factors. Recently, interest has increased in the potential use of stem cells for the repair of kidney injury, which has been considered as an alternative therapeutic strategy. Due to their high proliferation rates, their pluripotent differentiation potential, the finding that they do not induce tumour formation and the fact that they do not raise the ethical concerns connected with human embryonic stem cells, human amniotic fluid stem cells are considered to be a very promising cell source. This study demonstrates that the expression of the mesenchymal markers CD29 and CD44, the epithelial markers CD51 and ZO-1 and the podocyte markers CD2AP and NPHS2 can be induced in these cells via incubation with epidermal growth factor/platelet-derived growth factor BB and fibroblast growth factor 4/hepatocyte growth factor, respectively. Since podocytes are visceral epithelial cells in the kidneys, which form the essential part of the glomerular filtration barrier, these findings warrant further investigation of the potential use of human amniotic fluid stem cells for cell-based kidney therapies.
虽然透析和移植是广泛应用于肾衰竭的治疗方法，但发病率高、供体器官短缺和成本高等缺点限制了其应用。最近，人们对干细胞在肾脏损伤修复中的潜在应用产生了兴趣，这被认为是一种替代治疗策略。由于人羊膜间充质干细胞具有高增殖率、多能分化潜能、不诱导肿瘤形成以及不涉及与人类胚胎干细胞相关的伦理问题等特点，因此被认为是一种很有前途的细胞来源。本研究表明，通过分别用表皮生长因子/血小板衍生生长因子 BB 和成纤维细胞生长因子 4/肝细胞生长因子孵育人羊膜间充质干细胞，可以诱导其表达间充质标志物 CD29 和 CD44、上皮标志物 CD51 和 ZO-1 以及足细胞标志物 CD2AP 和 NPHS2。由于足细胞是肾脏中的内脏上皮细胞，是肾小球滤过屏障的重要组成部分，这些发现证明了人羊膜间充质干细胞在基于细胞的肾脏治疗中的潜在应用价值，值得进一步研究。
