Metabolic Research Centre, School of Medicine & Pharmacology, University of Western Australia, Perth, Western Australia 6847, Australia.
Atherosclerosis. 2010 May;210(1):326-30. doi: 10.1016/j.atherosclerosis.2009.11.024. Epub 2009 Nov 22.
The aim of this study was to investigate associations between plasma apoM concentration and HDL apoA-I and apoA-II kinetics in 60 overweight-obese, insulin resistant men.
Plasma apoM concentration was determined using a sandwich ELISA with two monoclonal antibodies (CV<5%). The kinetics of HDL apoA-I and apoA-II were measured using intravenous administration of D(3)-leucine, gas chromatography-mass spectrometry and multi-compartmental modeling.
Plasma apoM was inversely associated with body mass index and positively associated with plasma total cholesterol, LDL cholesterol and HDL cholesterol (p<0.05). There were no associations between plasma apoM and plasma triglyceride, NEFA, insulin, glucose, HOMA score or adiponectin concentrations. Plasma apoM was positively associated with both apoA-I and apoA-II concentrations (r=0.406, p<0.01 and r=0.510, p<0.01, respectively) and negatively associated with HDL apoA-I and apoA-II fractional catabolic rate (FCR) (r=-0.291, p=0.03 and r=-0.291, p=0.026, respectively). No significant associations were observed between plasma apoM and HDL apoA-I and apoA-II production rate. In multivariate regression models, both plasma apoM and triglycerides were significant, independent predictors of HDL apoA-I FCR (adjusted R(2)=16%, p<0.01) and HDL apoA-II FCR (adjusted R(2)=14%, p<0.01).
ApoM may be a significant, independent predictor of HDL apoA-I and apoA-II catabolism in overweight-obese, insulin resistant men.
本研究旨在探讨 60 名超重肥胖、胰岛素抵抗男性患者血浆载脂蛋白 M（apoM）浓度与高密度脂蛋白（HDL）载脂蛋白 A-I（apoA-I）和 apoA-II 代谢动力学之间的关系。
采用两种单克隆抗体（CV<5%）的夹心 ELISA 法测定血浆 apoM 浓度。采用静脉注射 D（3）-亮氨酸、气相色谱-质谱联用和多房室模型法测定 HDL apoA-I 和 apoA-II 的代谢动力学。
血浆 apoM 与体重指数呈负相关，与总胆固醇、LDL 胆固醇和 HDL 胆固醇呈正相关（p<0.05）。血浆 apoM 与甘油三酯、NEFA、胰岛素、血糖、HOMA 评分或脂联素浓度之间无相关性。血浆 apoM 与 apoA-I 和 apoA-II 浓度呈正相关（r=0.406，p<0.01 和 r=0.510，p<0.01），与 HDL apoA-I 和 apoA-II 分解代谢率（FCR）呈负相关（r=-0.291，p=0.03 和 r=-0.291，p=0.026，分别）。血浆 apoM 与 HDL apoA-I 和 apoA-II 产生率之间无显著相关性。在多元回归模型中，血浆 apoM 和甘油三酯均为 HDL apoA-I FCR（调整后的 R²=16%，p<0.01）和 HDL apoA-II FCR（调整后的 R²=14%，p<0.01）的显著独立预测因子。
apoM 可能是超重肥胖、胰岛素抵抗男性患者 HDL apoA-I 和 apoA-II 代谢的重要独立预测因子。
