Matthan Nirupa R, Jalbert Susan M, Lamon-Fava Stefania, Dolnikowski Gregory G, Welty Francine K, Barrett Hugh R, Schaefer Ernst J, Lichtenstein Alice H
Cardiovascular Nutrition, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington St, Boston, MA 02111, USA.
Arterioscler Thromb Vasc Biol. 2005 Aug;25(8):1691-6. doi: 10.1161/01.ATV.0000172629.12846.b8. Epub 2005 Jun 2.
To determine mechanisms contributing to the altered lipoprotein profile associated with aging and menopause, apolipoprotein B-100 (apoB-100) and apoA-I kinetic behavior was assessed.
Eight premenopausal (25+/-3 years) and 16 postmenopausal (65+/-6 years) women consumed for 6 weeks a standardized Western diet, at the end of which a primed-constant infusion of deuterated leucine was administered in the fed state to determine the kinetic behavior of triglyceride-rich lipoprotein (TRL), intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL) apoB-100, and high-density lipoprotein (HDL) apoA-I. Data were fit to a multicompartmental model using SAAM II to calculate fractional catabolic rate (FCR) and production rate (PR). Total cholesterol, LDL cholesterol (LDL-C), TRL-C, and triglyceride levels were higher (50%, 55%, 130%, and 232%, respectively) in the postmenopausal compared with the premenopausal women, whereas HDL-C levels were similar. Plasma TRL, IDL, and LDL-apoB-100 levels and pool sizes (PS) were significantly higher in the postmenopausal than premenopausal women. These differences were accounted for by lower TRL, IDL, and LDL apoB-100 FCR (P<0.05), with no difference in PR. There was no significant difference between groups in HDL-C levels or apoA-I kinetic parameters. Plasma TRL-C concentrations were negatively correlated with TRL apoB-100 FCR (r=-0.46; P<0.05) and positively correlated with PR (r=0.62; P<0.01). Plasma LDL-C concentrations were negatively correlated with LDL apoB-100 FCR (r=-0.70; P<0.001) but not PR.
The mechanism for the increase in TRL and LDL apoB-100 PS observed in the postmenopausal women was determined predominantly by decreased TRL and LDL catabolism rather than increased production. No differences were observed in HDL apoA-I kinetics between groups.
为确定与衰老和绝经相关的脂蛋白谱改变的机制，对载脂蛋白B - 100（apoB - 100）和载脂蛋白A - I（apoA - I）的动力学行为进行了评估。
8名绝经前女性（25±3岁）和16名绝经后女性（65±6岁）食用标准化西方饮食6周，在饮食结束时，于进食状态下进行氘代亮氨酸的单次静脉推注-持续输注，以确定富含甘油三酯脂蛋白（TRL）、中间密度脂蛋白（IDL）和低密度脂蛋白（LDL）的apoB - 100以及高密度脂蛋白（HDL）的apoA - I的动力学行为。使用SAAM II将数据拟合到多室模型，以计算分解代谢率（FCR）和生成率（PR）。与绝经前女性相比，绝经后女性的总胆固醇、低密度脂蛋白胆固醇（LDL - C）、TRL - C和甘油三酯水平更高（分别高出50%、55%、130%和232%），而高密度脂蛋白胆固醇（HDL - C）水平相似。绝经后女性的血浆TRL、IDL和LDL - apoB - 100水平及池大小（PS）显著高于绝经前女性。这些差异是由TRL、IDL和LDL的apoB - 100 FCR降低所致（P<0.05），而PR无差异。两组间HDL - C水平或apoA - I动力学参数无显著差异。血浆TRL - C浓度与TRL apoB - 100 FCR呈负相关（r = - 0.46；P<0.05），与PR呈正相关（r = 0.62；P<0.01）。血浆LDL - C浓度与LDL apoB - 100 FCR呈负相关（r = - 0.70；P<0.001），但与PR无关。
绝经后女性中观察到的TRL和LDL apoB - 100 PS增加的机制主要是由TRL和LDL分解代谢降低而非生成增加所致。两组间HDL apoA - I动力学无差异。
