State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Biosens Bioelectron. 2010 Mar 15;25(7):1609-14. doi: 10.1016/j.bios.2009.11.022. Epub 2009 Dec 2.
A novel method for selective collection and detection of human acute leukemia cells has been proposed using aptamer-conjugated magnetic beads (apt-MBs) and a magnet-quartz crystal microbalance (QCM) system. The sgc8c aptamer-conjugated MBs specifically binding to CCRF-CEM cells were used for target cell extraction from complex matrixes, and the magnet-QCM system was successfully applied for quantitative cell detection, requiring no further labeling of cells. The accumulation of MBs-conjugated CCRF-CEM cells on a quartz crystal gold electrode surface under a magnetic field resulted in decreased resonant frequency. A linear relationship between the frequency shift and cell concentration over the range of 1 x 10(4)-1.5 x 10(5)cells mL(-1) was obtained, with a detection limit of 8 x 10(3)cells mL(-1). The applicability of the method for target cell detection from cell mixture was satisfactory.
一种新的方法用于选择性收集和检测人急性白血病细胞已被提出使用适配体偶联磁珠（apt-MBs）和一个磁铁石英晶体微天平（QCM）系统。sgc8c 适配体偶联磁珠特异性结合到 CCRF-CEM 细胞被用于从复杂基质中提取靶细胞，和磁铁-QCM 系统成功地应用于定量细胞检测，不需要进一步标记细胞。磁珠偶联的 CCRF-CEM 细胞在磁场下在石英晶体金电极表面的积累导致共振频率降低。一个线性关系的频率位移和细胞浓度在 1 x 10(4)-1.5 x 10(5)细胞毫升(-1)范围内得到，检测限为 8 x 10(3)细胞毫升(-1)。该方法用于从细胞混合物中检测靶细胞的适用性是令人满意的。
