Institute of Animal Biochemistry and Genetics, Center of Biosciences SAS, Dúbravská cesta 9, 840 05 Bratislava, Slovakia.
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovakia.
Biosensors (Basel). 2019 May 27;9(2):72. doi: 10.3390/bios9020072.
Detection of the breast cancer cells is important for early diagnosis of the cancer. We applied thickness shear mode acoustics method (TSM) for detection of SK-BR-3 breast cancer cells using DNA aptamers specific to HER2 positive membrane receptors. The biotinylated aptamers were immobilized at the neutravidin layer chemisorbed at gold surface of TSM transducer. Addition of the cells resulted in decrease of resonant frequency, f, and in increase of motional resistance, R. Using gold nanoparticles (AuNPs), modified by aptamers it was possible improving the limit of detection (LOD) that reached 550 cells/mL, while without amplification the sensitivity of the detection of SK-BR-3 cells was 1574 cells/mL. HER2 negative cell line MDA-MB-231 did not resulted in significant changes of f. The viability studies demonstrated that cells are stable at experimental conditions used during at least 8 h. AuNPs were not toxic on the cells up to concentration of 1 μg/mL.
检测乳腺癌细胞对于癌症的早期诊断非常重要。我们应用厚度剪切模式声学方法（TSM），使用针对 HER2 阳性膜受体的 DNA 适体来检测 SK-BR-3 乳腺癌细胞。生物素化的适体被固定在化学吸附在 TSM 换能器金表面上的亲和素层上。添加细胞会导致共振频率 f 降低，运动阻力 R 增加。使用经过适体修饰的金纳米粒子（AuNPs），可以提高检测限（LOD），达到 550 个细胞/mL，而没有放大，SK-BR-3 细胞的检测灵敏度为 1574 个细胞/mL。HER2 阴性细胞系 MDA-MB-231 不会导致 f 发生显著变化。细胞活力研究表明，在实验条件下，细胞至少稳定 8 小时。AuNPs 的浓度高达 1μg/mL 时对细胞没有毒性。
